Vector-based attenuated poxvirus vaccines

Abstract

The present invention relates to a composition for raising an immune response in animal which decreases the risk of chikungunya and smallpox infection, Zika virus and smallpox infection, and/or chikungunya, Zika virus and smallpox infection. The composition comprises a pharmaceutically acceptable carrier and an attenuated poxvirus, wherein the poxvirus genome comprises a nucleic acid sequence encoding the 26S subgenomic polyprotein of chikungunya virus and/or the PrME of Zika virus.

1 . An immunogenic composition comprising a pharmaceutically acceptable carrier and an attenuated poxvirus, wherein the attenuated poxvirus genome comprises a nucleic acid sequence encoding the 26S subgenomic polyprotein of chikungunya virus and a nucleic acid sequence encoding the PrME polyprotein of Zika virus and further comprises deletion of at least one gene which encodes an endogenous essential assembly or maturation protein and/or increases immunogenicity of the composition. 
     
     
         2 . An immunogenic composition comprising a pharmaceutically acceptable carrier and an attenuated poxvirus, wherein the attenuated poxvirus genome comprises a nucleic acid sequence encoding the 26S subgenomic polyprotein of chikungunya virus and further comprises deletion of at least one gene which encodes an endogenous essential assembly or maturation protein and/or increases immunogenicity of the composition. 
     
     
         3 . An immunogenic composition comprising a pharmaceutically acceptable carrier and an attenuated poxvirus, wherein the attenuated poxvirus genome comprises a nucleic acid sequence encoding the PrME polyprotein of Zika virus and further comprises deletion of at least one gene which encodes an endogenous essential assembly or maturation protein and/or increases immunogenicity of the composition. 
     
     
         4 . The immunogenic composition of  claim 1 , wherein the attenuated poxvirus is selected from the group consisting of vaccinia, cowpox, Modified Vaccinia Ankara (MVA), NYVAC, avipox, canarypox and fowlpox. 
     
     
         5 . The immunogenic composition of  claim 1 , wherein the gene which encodes an endogenous essential assembly or maturation protein and/or increases immunogenicity of the composition is a poxvirus D13L gene. 
     
     
         6 . The immunogenic composition of  claim 1 , wherein gene which encodes an endogenous essential assembly or maturation protein and/or increases immunogenicity of the composition is a poxvirus K1L gene. 
     
     
         7 . The immunogenic composition of  claim 1 , wherein the gene which encodes an endogenous essential assembly or maturation protein and/or increases immunogenicity of the composition is a poxvirus A39R gene. 
     
     
         8 . The immunogenic composition of  claim 1 , wherein the genes which encode an endogenous essential assembly or maturation protein and/or increases immunogenicity of the composition are poxvirus B7R-B8R genes. 
     
     
         9 . The immunogenic composition of  claim 1 , wherein the attenuated poxvirus genome comprises a deletion in the poxvirus D13L gene, the A39R gene and the B7R-B8R genes. 
     
     
         10 . The immunogenic composition of  claim 1 , wherein the pharmaceutically acceptable carrier comprises an adjuvant. 
     
     
         11 . The immunogenic composition of  claim 10 , wherein the adjuvant is selected from the group consisting of aluminium hydroxide, aluminium phosphate, potassium aluminium sulfate, calcium phosphate hydroxide, Freund's complete adjuvant, Freund's incomplete adjuvant, iscoms, and iscomatrix. 
     
     
         12 . A method of inducing a protective immune response in a subject against chikungunya and smallpox virus infection, smallpox and Zika virus infection and/or chikungunya, smallpox and Zika virus infection the method comprising administering to the subject the immunogenic composition of  claim 1 .