Methods for manufacuring live lactobacillus cells

Abstract

Lactobacillus-based probiotic compositions for inclusion in microbial-based products. In one instance, the microbial-based product takes the form of an animal feed additive. When used as a feed additive for animals, especially ruminants, and in particular cattle, the following benefits from the composition are noted: (i) improved milk yield in dairy cattle, (ii) improved milk composition in dairy cattle, (iii) increased weight gain-to-feed intake ratio in beef cattle (feed efficiency), and (vi) improved average daily weight gain. Another marked benefit of the compositions when used as an animal feed additive is inhibition of pathogenic growth including E. coli, Salmonella, Clostridium, Staphylococcus and Streptococcus.

1 . A method of manufacturing live bacterial cells comprising:
 inoculating a bacterial fermentation medium with live  Lactobacillus  cells strain M35 having ATCC Accession Number PTA-5249; and   harvesting the live  Lactobacillus  cells in the logarithmic phase of growth in which the  Lactobacillus  cells are substantially spherically shaped.   
     
     
         2 . The method as recited in  claim 1 , further comprising:
 inoculating a bacterial fermentation medium with live  Propionibacterium  cells; and   harvesting the live  Propionibacterium  cells.   
     
     
         3 . The method as recited in  claim 2 , wherein the  Propionibacterium  cells are one of  Propionibacterium freudenreichiim  cells and  Propionibacterium acidipropionici  cells. 
     
     
         4 . The method as recited in  claim 1 , further comprising:
 harvesting the live  Lactobacillus  cells during at least one logarithmic phase of growth.   
     
     
         5 . The method as recited in  claim 1 , further comprising:
 harvesting the live  Lactobacillus  cells at least partially during at least one of (i) mid-log phase of growth and (ii) late-log phase of growth.   
     
     
         6 . The method as recited in  claim 5 , further comprising:
 harvesting the live  Lactobacillus  cells during mid-log phase of growth.   
     
     
         7 . The method as recited in  claim 5 , further comprising:
 harvesting the live  Lactobacillus  cells during late-log phase of growth.   
     
     
         8 . The method as recited in  claim 1 , further comprising:
 harvesting the live  Lactobacillus  cells between mid-log phase of growth and late-log phase of growth.   
     
     
         9 . The method as recited in  claim 1 , further comprising:
 harvesting the live  Lactobacillus  cells at least partially during mid-log phase of growth and at least partially during another log phase of growth.   
     
     
         10 . The method as recited in  claim 1 , further comprising:
 harvesting the live  Lactobacillus  cells when the live cells are spherically shaped.   
     
     
         11 . A method of manufacturing live bacterial cells comprising:
 inoculating a bacterial fermentation medium with live  Lactobacillus  cells strain M35 having ATCC Accession Number PTA-5249 and having a homology of 99.79% to  L. amylovorus ; and   harvesting the live  Lactobacillus  cells in the logarithmic phase of growth in which the  Lactobacillus  cells are substantially spherically shaped.   
     
     
         12 . The method as recited in  claim 11 , further comprising:
 inoculating a bacterial fermentation medium with live  Propionibacterium  cells; and   harvesting the live  Propionibacterium  cells.   
     
     
         13 . The method as recited in  claim 12 , wherein the  Propionibacterium  cells are one of  Propionibacterium freudenreichiim  cells and  Propionibacterium acidipropionici  cells. 
     
     
         14 . The method as recited in  claim 11 , further comprising:
 harvesting the live  Lactobacillus  cells during at least one logarithmic phase of growth.   
     
     
         15 . The method as recited in  claim 11 , further comprising:
 harvesting the live  Lactobacillus  cells at least partially during at least one of (i) mid-log phase of growth and (ii) late-log phase of growth.   
     
     
         16 . The method as recited in  claim 15 , further comprising:
 harvesting the live  Lactobacillus  cells during mid-log phase of growth.   
     
     
         17 . The method as recited in  claim 15 , further comprising:
 harvesting the live  Lactobacillus  cells during late-log phase of growth.   
     
     
         18 . The method as recited in  claim 11 , further comprising:
 harvesting the live  Lactobacillus  cells between mid-log phase of growth and late-log phase of growth.   
     
     
         19 . The method as recited in  claim 11 , further comprising:
 harvesting the live  Lactobacillus  cells at least partially during mid-log phase of growth and at least partially during another log phase of growth.   
     
     
         20 . The method as recited in  claim 11 , further comprising:
 harvesting the live  Lactobacillus  cells when the live cells are spherically shaped.