Rapid Measurement of Formed Blood Component Sedimentation Rate from Small Sample Volumes

Abstract

Devices and methods are described for measuring formed blood component sedimentation rate. Some of the methods may use (1) centrifugal techniques for separating red blood cells from plasma and (2) video and/or still imaging capability. Both may be used alone or in combination to accelerate formed blood component sedimentation and to measure its rate. In one example, the method may advantageously enable rapid measurement of sedimentation rate using small blood sample volumes. Automated image analysis can be used to determine both sedimentation rate and hematocrit. Automated techniques may be used to compensate for effects of hematocrit on uncorrected sedimentation rate data.

1 - 39 . (canceled) 
     
     
         40 . A method comprising:
 collecting a plurality of images of formed blood component and plasma interface positions over time from an accelerated blood sample compaction process;   performing image transformation on said plurality of images to transform images with curved interfaces into corrected images with straight line interfaces;   establishing a time-related compaction curve based on interface positions in said corrected images, for at least one formed blood component in said blood sample.   
     
     
         41 . (canceled) 
     
     
         42 . A method comprising:
 using a programmable processor-controlled system to transfer at least a portion of a blood sample from a blood sample location into a centrifugation vessel;   using a sample handling system under programmable processor control to transfer said vessel from a first addressable position to a centrifuge with a second addressable position;   centrifuging the blood sample in the vessel for a period of time;   collecting a plurality of images of formed blood component and plasma interface positions over time;   establishing a time-related compaction curve based on interface positions in said corrected images, for at least one formed blood component in said blood sample after centrifuging has begun.   
     
     
         43 . The method of  claim 42 , wherein the centrifuge has a rotor with a diameter of about 15 cm or less. 
     
     
         44 . The method of  claim 42 , wherein the centrifuge has a rotor with a diameter of about 10 cm or less. 
     
     
         45 . The method of  claim 42 , wherein the centrifuge has a rotor when in motion circumscribes an area with a longest dimension of about 15 cm or less. 
     
     
         46 . The method of  claim 42 , wherein the centrifuge has a rotor when in motion circumscribes an area with a longest dimension of about 10 cm or less. 
     
     
         47 - 51 . (canceled) 
     
     
         52 . A system comprising:
 a centrifuge having a centrifuge vessel holder configured to allow for detection of blood component interface position in the vessel holder in the vessel holder during centrifugation;   a sample handling system for transporting a blood sample from a first location to a location on the centrifuge; and   a processor programmed to record interface position during a least a portion of centrifugation.   
     
     
         53 . The system of  claim 52 , wherein the centrifuge has window to allow for visual observation of a centrifuge vessel holder during centrifugation. 
     
     
         54 . The system of  claim 52 , wherein the centrifuge an illumination source to allow for detection of blood component interface position in the sample.