Liquid chromatography technique

Abstract

Liquid chromatography techniques are disclosed. Specifically, the liquid chromatography technique includes providing a liquid chromatography system having a coated metallic fluid-contacting element, and transporting a fluid to contact the coated metallic fluid-contacting element. Conditions for the transporting of the fluid are selected from the group consisting of the temperature of the fluid being greater than 150° C., pressure urging the fluid being greater than 60 MPa, the fluid having a protein-containing analyte incompatible with one or both of titanium and polyether ether ketone, the fluid having a chelating agent incompatible with the one or both of the titanium or the polyether ether ketone, and combinations thereof

What is claimed is: 
     
         1 . A liquid chromatography technique, comprising:
 providing a liquid chromatography system having a coated stainless steel fluid-contacting element; and   transporting a fluid to contact the coated stainless steel fluid-contacting element;   wherein the fluid includes adenosine triphosphate;   wherein the coated stainless steel fluid-contacting element has a coating, the coating including carbon, silicon, oxygen, and hydrogen.   
     
     
         2 . The technique of  claim 1 , wherein the coated stainless steel fluid-contacting element is a frit. 
     
     
         3 . The technique of  claim 1 , wherein the coated stainless steel fluid-contacting element is a fitting. 
     
     
         4 . The technique of  claim 1 , wherein the coated stainless steel fluid-contacting element is a pump head. 
     
     
         5 . The technique of  claim 1 , wherein the coated stainless steel fluid-contacting element is a valve. 
     
     
         6 . The technique of  claim 1 , wherein the liquid chromatography system has a stationary phase, the stationary phase having particles having a size of less than 6 micrometers. 
     
     
         7 . The technique of  claim 1 , wherein the liquid chromatography system has a stationary phase, the stationary phase having particles being hydrophobic. 
     
     
         8 . The technique of  claim 1 , wherein the liquid chromatography system includes a solvent reservoir, a solvent degasser, a gradient valve, a mixing vessel, a high-pressure pump, switching valves, a sample injection loop, a pre-column portion, a coated metallic column, a detector, a data acquisition system, and a waste collector. 
     
     
         9 . The technique of  claim 1 , wherein the liquid technique is high performance liquid chromatography. 
     
     
         10 . The technique of  claim 1 , wherein the liquid technique is ultra high performance liquid chromatography. 
     
     
         11 . The technique of  claim 1 , wherein the liquid technique is partitioned high performance liquid chromatography. 
     
     
         12 . The technique of  claim 1 , wherein the liquid technique is partitioned ultra high performance liquid chromatography. 
     
     
         13 . The technique of  claim 1 , wherein the liquid technique is normal-phase chromatography. 
     
     
         14 . The technique of  claim 1 , wherein the liquid technique is displacement chromatography. 
     
     
         15 . The technique of  claim 1 , wherein the liquid technique is reversed-phase chromatography. 
     
     
         16 . The technique of  claim 1 , wherein the liquid technique is size exclusion chromatography. 
     
     
         17 . The technique of  claim 1 , wherein the liquid technique is ion-exchange chromatography. 
     
     
         18 . A liquid chromatography technique, comprising:
 transporting adenosine triphosphate in a liquid chromatography system, the liquid chromatography having a frit, a fitting, a valve, and a pump head;   wherein the at least one of the frit, the fitting, the valve, and the pump head have a coating, the coating including carbon, silicon, oxygen, and hydrogen.   
     
     
         19 . The technique of  claim 18 , wherein each of the frit, the fitting, the valve, and the pump head have the coating. 
     
     
         20 . A liquid chromatography technique, comprising:
 providing a liquid chromatography system having a coated stainless steel fluid-contacting element; and   transporting a fluid to contact the coated stainless steel fluid-contacting element;   wherein the fluid includes tetracycline.