Bioprinting platform, method for using the same and 3d bioprinter

Abstract

A device and method for printing lumen tissue constructs and a 3D bioprinter are disclosed. The device has a spray sprayhead assembly and a bioprinting platform. The spray sprayhead assembly prints a biological construct on an inner surface of a lumen tissue by the bioprinting platform. Problems, such as, recurrence of thrombus and restenosis of a lumen after the lumen tissue has been transplanted for a long time, can be avoided. The biological reliability of the lumen tissue can be improved.

1 - 26 . (canceled) 
     
     
         27 . The bioprinting platform comprising:
 a platform base;   a rotary part on the platform base, comprising a rotary rod for placing a bio-block and a medical adhesive to form a biological construct; and   a butt-jointed part movable relative to the rotary part, comprising a hollow rod having an outer wall for placing lumen tissue.   
     
     
         28 . The bioprinting platform of  claim 27 , wherein the outer wall of the rotary rod is covered with an elastic film. 
     
     
         29 . The bioprinting platform of  claim 28 , wherein an interior of the rotary rod is hollow, and the outer wall of the rotary rod is provided with a vent communicating with the interior, for exhausting air inside the rotary rod to balloon the elastic film. 
     
     
         30 . The bioprinting platform of  claim 27 , wherein an interior of the rotary rod is provided with a heating unit. 
     
     
         31 . The bioprinting platform of  claim 30 , wherein the heating unit includes a heating section and a spacing section that are spacedly arranged, the heating section has a surface wound with a resistance wire, and the heating section has a diameter that is less than that of the spacing section. 
     
     
         32 . The bioprinting platform of  claim 30 , wherein a temperature detecting unit is provided at one end of the heating unit proximate to the butt-jointed part, for detecting a temperature of the heating unit. 
     
     
         33 . The bioprinting platform of  claim 27 , wherein the bioprinting platform includes a gripping mechanism for gripping the lumen tissue to make the lumen tissue disengaged from the hollow rod and socketed to the biological construct. 
     
     
         34 . The bioprinting platform of  claim 33 , wherein the gripping mechanism includes a first gripping block and a second gripping block which are movable relatively. 
     
     
         35 . The bioprinting platform of  claim 34 , wherein the gripping mechanism includes a retaining unit for acting on a tail end of the lumen tissue so that the lumen tissue is disengaged from the hollow rod. 
     
     
         36 . The bioprinting platform of  claim 35 , wherein the retaining unit is cooperatively provided with a retaining ring acting on the tail end of the lumen tissue. 
     
     
         37 . The bioprinting platform of  claim 34 , wherein the gripping mechanism includes a limiting block provided at a bottom of the first gripping block and the second gripping block, for limiting relative movement of the first gripping block and the second gripping block, so that the first gripping block and the second gripping block are both tangent to an outer wall of the lumen tissue. 
     
     
         38 . The bioprinting platform of  claim 34 , wherein the gripping mechanism includes a support platform provided at a bottom of the first gripping block and the second gripping block, for supporting the lumen tissue. 
     
     
         39 . The bioprinting platform of  claim 27 , further comprising an optical probe movable inside the rotary rod, for detecting a flatness of an inner wall of the biological construct, wherein the rotary rod is made of a transparent material. 
     
     
         40 . The bioprinting platform of  claim 39 , wherein the optical probe is movably disposed within the rotary rod or the hollow rod. 
     
     
         41 . The bioprinting platform of  claim 39 , wherein the optical probe is fixedly disposed within the hollow rod. 
     
     
         42 . The bioprinting platform of  claim 33 , further comprising a reservoir provided below the rotary rod, for bearing a bioprinting construct disengaged and falling from the gripping mechanism. 
     
     
         43 . A 3D bioprinter comprising the bioprinting platform of  claim 27 . 
     
     
         44 . A method of printing lumen tissue construct using the bioprinting platform of  claim 27 , comprising a mantling step comprising: cladding a layer of elastic film on an outer wall of the rotary rod before printing the biological construct. 
     
     
         45 . The method of  claim 44 , further comprising a ballooning step comprising: ventilating into the layer of elastic film to balloon the layer of elastic film so that the biological construct is attached to the inner surface of the lumen tissue, after the lumen tissue is sleeved outside the biological construct.