Synthetic peptide sp4 and use thereof

Abstract

A synthetic peptide sp4 has an amino acid sequence shown in SEQ ID NO: 1. As the sole effective ingredient in the antitumor efficacy test, it has a significant inhibitory effect on both the tumor volume and tumor weight of human osteosarcoma MG-63 in nude mice and has a significant dose-effect and time-effect relationship. There is no difference between the relative tumor growth rate T/C (%) of sp4 and that of the positive control group of Paclitaxel. The safety of sp4 is that its maximal tolerated dose for intravenous administration is 700 mg/kgBW. Sp4 has clear targets of pharmacological effects, while having efficacy in vivo, it also has an inhibitory effect on tumor telomerase, has an arrest on G1 phase of the tumor cell cycle, and inhibits the high expressions of tumor PD-L1 and CD47. The present disclosure relates to multiple uses of sp4, especially in the preparation of a class of antitumor drugs.

1 . A synthetic peptide sp4, having an amino acid sequence as shown in SEQ ID NO: 1. 
     
     
         2 . Uses of the synthetic peptide sp4 according to  claim 1 , comprising: (1) preventing and/or treating tumors; (2) inhibiting proliferation and/or growth and/or invasion of tumor cells; (3) enhancing anti-tumor immunoreaction; (4) inducing differentiation of tumor cells; (5) preparing anti-tumor drugs; (6) inhibiting the activity of tumor telomerase; (7) regulating tumor cell cycle; and (8) preparing products for regulating tumor cell cycle. 
     
     
         3 . Uses of the synthetic peptide sp4 according to  claim 2 , wherein the tumor according to any one of (1) to (8) is human osteosarcoma or human esophagus cancer. 
     
     
         4 . Uses of the synthetic peptide sp4 according to  claim 3 , wherein the human esophagus cancer comprises malignant tumors of esophageal squamous epithelium and columnar epithelium. 
     
     
         5 . A biologically active molecular product, wherein the active ingredient thereof comprises the synthetic peptide sp4 according to  claim 1 . 
     
     
         6 . A biologically active molecular product, wherein the sole effective active ingredient thereof is the synthetic peptide sp4 according to  claim 1 .