Polypeptides having transgalctosylating activity

Abstract

The present invention relates to polypeptides, specifically polypeptides having transgalactosylating activity and nucleic acids encoding these, and their uses in e.g. dairy product.

1 . A polypeptide having transgalactosylating activity selected from the group consisting of:
 a. a polypeptide comprising an amino acid sequence having at least 90% sequence identity with SEQ ID NO: 1, wherein said polypeptide consists of at most 950, 940, 930, 920, 910, 900, or 890 amino acid residues.   
     
     
         2 . The polypeptide according to  claim 1  comprising an amino acid sequence having at least 90% sequence identity with SEQ ID NO: 1, wherein said polypeptide consists of at most 890 amino acid residues. 
     
     
         3 . (canceled) 
     
     
         4 . (canceled) 
     
     
         5 . The polypeptide according to  claim 1  which consists of the amino acid sequence of SEQ ID NO: 1. 
     
     
         6 . (canceled) 
     
     
         7 . The polypeptide according to  claim 1 , wherein said polypeptide has a ratio of transgalactosylation activity above 100%. 
     
     
         8 . A nucleic acid capable of encoding a polypeptide according to  claim 1 . 
     
     
         9 . An expression vector comprising a nucleic acid according to  claim 8 . 
     
     
         10 . A cell capable of expressing a polypeptide according to  claim 1 . 
     
     
         11 . A method of expressing a polypeptide, the method comprising obtaining a cell according  claim 10  and expressing the polypeptide from the cell, and optionally purifying the polypeptide. 
     
     
         12 . A composition comprising a polypeptide according to  claim 1 , preferably a food composition, more preferably a dairy product. 
     
     
         13 . A method for producing a food product by treating a substrate comprising lactose with a polypeptide according to  claim 1 . 
     
     
         14 . A method for producing a dairy product by treating a milk-based substrate comprising lactose with a polypeptide according to  claim 1 . 
     
     
         15 . A process for producing galacto-oligosaccharides, comprising contacting of a polypeptide of according to  claim 1  with a milk-based solution comprising lactose.