Nucleotide analogs

Abstract

Provided herein is technology relating to the manipulation and detection of nucleic acids, including but not limited to compositions, methods, and kits related to nucleotides comprising a chemically reactive linking moiety.

We claim: 
     
         1 . A composition comprising a nucleotide analog having a structure according to: 
       
         
           
           
               
               
           
         
         wherein B is a base and P comprises a phosphate moiety. 
       
     
     
         2 . The composition of  claim 1  wherein P comprises a tetraphosphate, a triphosphate, a diphosphate, a monophosphate, a modified tetraphosphate, a modified triphosphate, a modified diphosphate, or a modified monophosphate. 
     
     
         3 . The composition of  claim 1  wherein B is selected from the group consisting of cytosine, guanine, adenine, thymine, and uracil. 
     
     
         4 . The composition of  claim 1  wherein B comprises a purine, a pyrimidine, a modified purine, or a modified pyrimidine 
     
     
         5 . The composition of  claim 1  wherein the nucleotide analog comprises a thio-alkynyl, thio-propargyl, 3′-S-propargyl, thiofuranose, thioribose, thiodeoxyribose, arabinose, or a modified sugar. 
     
     
         6 . The composition of  claim 1  wherein P comprises a 5′ hydroxyl, an alpha thiophosphate, a beta thiophosphate, a gamma thiophosphate, an alpha methylphosphonate, a beta methylphosphonate, or a gamma methylphosphonate. 
     
     
         7 . The composition of  claim 1  further comprising a polymerase, a nucleic acid, or a nucleotide. 
     
     
         8 . The composition of  claim 1  wherein the nucleotide analog is modified with a sulfur. 
     
     
         9 . The composition of  claim 1  further comprising a nucleotide comprising the base B, wherein the number ratio of the nucleotide analog to the nucleotide is 1:1, 1:2, 1:3, 1:4, 1:5, 1:10, 1:15, 1:20, 1:25, 1:30, 1:50, 1:75, 1:100, 1:200, 1:300, 1:400, 1:500, 1:600, 1:700, 1:800, 1:900, 1:1000, 1:5000 or 1:10000. 
     
     
         10 . A composition comprising a nucleic acid comprising the nucleotide analog of  claim 1 . 
     
     
         11 . The composition of  claim 10  wherein the nucleotide analog is at the 3′ end of the nucleic acid. 
     
     
         12 . The composition of  claim 10  wherein the nucleic acid is produced by a polymerase. 
     
     
         13 . The composition of  claim 10  further comprising an azide. 
     
     
         14 . The composition of  claim 10  further comprising a second nucleic acid. 
     
     
         15 . The composition of  claim 10  further comprising a second nucleic acid comprising an azide moiety. 
     
     
         16 . The composition of  claim 10  further comprising a second nucleic acid comprising an azide moiety at the 5′ end of the second nucleic acid. 
     
     
         17 . The composition of  claim 10  further comprising a label comprising an azide, a tag comprising an azide, a solid support comprising an azide, a nucleotide comprising an azide, a biotin comprising an azide, or a protein comprising an azide. 
     
     
         18 . The composition of  claim 10  further comprising a copper-based catalyst reagent. 
     
     
         19 . The composition of  claim 10  further comprising a nucleic acid comprising a triazole. 
     
     
         20 . The composition of  claim 10  further comprising a nucleic acid comprising a 1′, 4′ substituted triazole. 
     
     
         21 . The composition of  claim 10  further comprising an adaptor oligonucleotide, an adaptor oligonucleotide comprising a barcode, or a barcode oligonucleotide. 
     
     
         22 . The composition of  claim 10  further comprising a nucleic acid comprising a structure according to: 
       
         
           
           
               
               
           
         
       
     
     
         23 . A composition comprising a 3′O-propargyl nucleotide and a 5′-oligonucleotide