Assay analysis

Abstract

Apparatus and assemblies for the detection of at least one analyte in a sample are shown and described. In one embodiment, the assembly generates a test result from an assay and includes an integrated reader and incubator, wherein the incubator incubates the assay as the reader generates the test result. The reader typically has an optical detector aligned with a light source for detecting a plurality of transmission of light on the assay. The result is systems and methods to improve the detection of the presence and/or absence of at least one analyte in a sample.

What is claimed is: 
     
         1 . An apparatus to generate a test result from an assay when contacted with a sample, said apparatus comprising:
 a. an incubator adapted to incubate said assay; and   b. a fluorescence detector adapted to detect a first fluorescence on said assay and adapted to detect at least a subsequent fluorescence on said assay, and
 wherein incubation of said assay and detection of said fluorescence on said assay generates said test result. 
   
     
     
         2 . The apparatus of  claim 1 , wherein said apparatus is adapted to perform a continuous fluorescence detection of said assay. 
     
     
         3 . The apparatus of  claim 1 , wherein an incubation environment comprises a heated environment. 
     
     
         4 . The apparatus of  claim 1 , wherein an incubation environment comprises a cooled environment. 
     
     
         5 . The apparatus of  claim 1 , wherein an incubation environment comprises a maintained consistent temperature environment. 
     
     
         6 . The apparatus of  claim 1 , wherein said fluorescence detector detects a fluorescence on said assay. 
     
     
         7 . The apparatus of  claim 1 , wherein said fluorescence detector monitors at least one pre-test parameter after acquiring at least one fluorescence detection on said assay. 
     
     
         8 . The apparatus of  claim 7 , wherein said fluorescence detection corresponds to an optical reflectance value. 
     
     
         9 . The apparatus of  claim 1 , wherein said assay is a test strip having at least one test line and at least one control line, and whereby a theoretical reflectance value is a comparison between a reflectance value at said test line and a reflectance value at said control line. 
     
     
         10 . The apparatus of  claim 1 , wherein said incubator is a temperature adjustable incubator comprising at least one temperature control. 
     
     
         11 . The apparatus of  claim 1 , further including a housing adapted to substantially enclose said reader and said incubator. 
     
     
         12 . The apparatus of  claim 1 , further including a user interface having a display board. 
     
     
         13 . An assembly to generate a test result from an assay, said assembly comprising:
 a. a fluorometer adapted to read fluorescence on said assay and adapted to read a subsequent fluorescence on said assay; and   b. an incubator adapted to incubate said assay.   
     
     
         14 . The assembly of  claim 13 , wherein said assembly includes a housing substantially enclosing said incubator and said fluorometer. 
     
     
         15 . The assembly of  claim 13 , wherein said assembly performs continuous fluorescence detection of said assay to generate said test result. 
     
     
         16 . In an incubated apparatus to generate a test result from an assay when contacted with a sample, a reader comprising:
 a. a fluorescence detector adapted to analyze a first fluorescence on said assay and adapted to analyze a plurality of subsequent fluorescence on said assay, and
 wherein incubation of said assay and analyzing of said fluorescence on said assay generates said test result. 
   
     
     
         17 . The apparatus of  claim 16 , including a first incubation environment comprising a heated environment. 
     
     
         18 . The apparatus of  claim 16 , including a second incubation environment comprising a cooled environment. 
     
     
         19 . The apparatus of  claim 16 , wherein said incubator receives and said reader detects fluorescence on a test strip. 
     
     
         20 . The assembly of  claim 16 , wherein said reader performs continuous detection of fluorescence of said assay to generate said test result.