Resistance alleles in soybean

Abstract

The present invention relates to methods and compositions for identifying, selecting and/or producing a soybean plant or germplasm having resistance Phakosora pachyrhizi . A soybean plant, part thereof and/or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided. Also provided are single nucleotide polymorphisms (SNPs) associated with resistance to pathogens; and compositions including amplification primer pairs capable of initiating DNA polymerization by a DNA polymerase on soybean nucleic acid templates to generate soybean marker amplicons.

That which is claimed: 
     
         1 . A method of identifying an Asian Soy Rust (ASR) tolerant soybean plant or part thereof, comprising the steps of:
 a. isolating a nucleic acid from a soybean plant or part thereof;   b. detecting in said nucleic acid, the presence of a marker associated with ASR tolerance in a soybean plant, wherein said marker is located within a chromosomal interval on soybean chromosome 18 corresponding to physical positions 58,722,971-60,910,083; and   c. thereby identifying a ASR tolerant soybean plant or part thereof.   
     
     
         2 . The method of  claim 1 , wherein the chromosomal interval comprises an allele associated with ASR tolerance wherein said allele corresponds to both of an A at position 428 of SEQ ID NO: 1 and an A at position 806 of SEQ ID NO: 2. 
     
     
         3 . The method of  claim 2 , wherein the chromosomal interval further comprises an allele associated with ASR tolerance wherein said allele corresponds to any of an A at position 895 of SEQ ID NO: 3; a G at position 300 of SEQ ID NO: 4; a T at position 301 of SEQ ID NO: 5; an A at position 300 of SEQ ID NO: 6; an A at position 301 of SEQ ID NO: 7; or any combination thereof. 
     
     
         4 . The method of  claim 1 , wherein the soybean plant is an elite soybean plant. 
     
     
         5 . The method of  claim 1 , wherein the chromosomal interval is derived from any of PI200492, PI368038, PI368039, PI547875, PI594754, PI594760B, PI594767A, PI587880A, PI587905, PI561356, PI594538A, and a progeny thereof. 
     
     
         6 . An ASR tolerant soybean plant identified by the method of  claim 1 . 
     
     
         7 . An ASR tolerant progeny plant of the ASR tolerant soybean plant of  claim 6 , the progeny plant having the marker associated with ASR tolerance introduced into its genome from the ASR tolerant soybean plant via a breeding program.