US2021268095A1PendingUtilityA1

Escherichia coli compositions and methods thereof

59
Assignee: PFIZERPriority: Feb 23, 2020Filed: Feb 22, 2021Published: Sep 2, 2021
Est. expiryFeb 23, 2040(~13.6 yrs left)· nominal 20-yr term from priority
Inventors:Robert G. K. DonaldRosalind Pan
C07K 14/26C07K 14/245A61P 31/04A61K 2039/6037A61K 47/646A61K 47/6415A61K 39/0266A61K 39/0258A61P 37/04A61K 2039/6031A61K 2039/55577A61K 2039/55566A61K 2039/55505A61K 38/00A61K 35/74A61K 2039/545A61K 39/385Y02A50/30
59
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

This invention provides a polypeptide derived from E. coli or a fragment thereof, including compositions and methods thereof. In one embodiment, the compositions comprise a polypeptide derived from E. coli or a fragment thereof; and modified O-polysaccharide molecules derived from E. coli lipopolysaccharides or conjugates thereof. In a further aspect, the compositions further comprise modified O-polysaccharide molecules derived from Klebsiella pneumoniae or conjugates thereof.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 . A composition comprising a polypeptide derived from FimH or a fragment thereof; and a saccharide comprising a structure selected from any one of Formula O1, Formula O1A, Formula O1B, Formula O1C, Formula O2, Formula O3, Formula O4, Formula O4:K52, Formula O4:K6, Formula O5, Formula O5ab, Formula O5ac, Formula O6, Formula O6:K2; K13; K15, Formula O6:K54, Formula O7, Formula O8, Formula O9, Formula O10, Formula O11, Formula O12, Formula O13, Formula O14, Formula O15, Formula O16, Formula O17, Formula O18, Formula O18A, Formula O18ac, Formula O18A1, Formula O18B, Formula O18B1, Formula O19, Formula O20, Formula O21, Formula O22, Formula O23, Formula O23A, Formula O24, Formula O25, Formula O25a, Formula O25b, Formula O26, Formula O27, Formula O28, Formula O29, Formula O30, Formula O32, Formula O33, Formula O34, Formula O35, Formula O36, Formula O37, Formula O38, Formula O39, Formula O40, Formula O41, Formula O42, Formula O43, Formula O44, Formula O45, Formula O45, Formula O45rel, Formula O46, Formula O48, Formula O49, Formula O50, Formula O51, Formula O52, Formula O53, Formula O54, Formula O55, Formula O56, Formula O57, Formula O58, Formula O59, Formula O60, Formula O61, Formula O62, Formula 62D 1 , Formula O63, Formula O64, Formula O65, Formula O66, Formula O68, Formula O69, Formula O70, Formula O71, Formula O73, Formula O73, Formula O74, Formula O75, Formula O76, Formula O77, Formula O78, Formula O79, Formula O80, Formula O81, Formula O82, Formula O83, Formula O84, Formula O85, Formula O86, Formula O87, Formula O88, Formula O89, Formula O90, Formula O91, Formula O92, Formula O93, Formula O95, Formula O96, Formula O97, Formula O98, Formula O99, Formula O100, Formula O101, Formula O102, Formula O103, Formula O104, Formula O105, Formula O106, Formula O107, Formula O108, Formula O109, Formula O110, Formula O111, Formula O112, Formula O113, Formula O114, Formula O115, Formula O116, Formula O117, Formula O118, Formula O119, Formula O120, Formula O121, Formula O123, Formula O124, Formula O125, Formula O126, Formula O127, Formula O128, Formula O129, Formula O130, Formula O131, Formula O132, Formula O133, Formula O134, Formula O135, Formula O136, Formula O137, Formula O138, Formula O139, Formula O140, Formula O141, Formula O142, Formula O143, Formula O144, Formula O145, Formula O146, Formula O147, Formula O148, Formula O149, Formula O150, Formula O151, Formula O152, Formula O153, Formula O154, Formula O155, Formula O156, Formula O157, Formula O158, Formula O159, Formula O160, Formula O161, Formula O162, Formula O163, Formula O164, Formula O165, Formula O166, Formula O167, Formula O168, Formula O169, Formula O170, Formula O171, Formula O172, Formula O173, Formula O174, Formula O175, Formula O176, Formula O177, Formula O178, Formula O179, Formula O180, Formula O181, Formula O182, Formula O183, Formula O184, Formula O185, Formula O186, Formula O187. 
     
     
         2 . The composition according to  claim 1 , further comprising at least one saccharide derived from any one  K. pneumoniae  type selected from the group consisting of O1, O2, O3, and O5. 
     
     
         3 . The composition according to  claim 1 , further comprising a saccharide derived from  Klebsiella pneumoniae  type O1. 
     
     
         4 . The composition according to  claim 1 , further comprising a saccharide derived from  K. pneumoniae  type O2. 
     
     
         5 . The composition according to  claim 1 , further comprising a saccharide derived from  K. pneumoniae  type O3. 
     
     
         6 . The composition according to  claim 1 , further comprising a saccharide derived from  K. pneumoniae  type O5. 
     
     
         7 . The composition according to  claim 1 , further comprising a saccharide derived from  K. pneumoniae  type O1 and a saccharide derived from  K. pneumoniae  type O2. 
     
     
         8 . The composition according to  claim 2 , wherein the saccharide derived from  K. pneumoniae  is conjugated to a carrier protein; and the saccharide derived from  E. coli  is conjugated to a carrier protein. 
     
     
         9 . The composition according to  claim 1 , further comprising a polypeptide derived from  K. pneumoniae.    
     
     
         10 . A composition comprising a polypeptide derived from FimH or a fragment thereof; and at least one saccharide derived from any one  K. pneumoniae  type selected from the group consisting of O1, O2, O3, and O5. 
     
     
         11 . The composition according to  claim 10 , further comprising at least one saccharide comprising a structure selected from any one of Formula O1, Formula O1A, Formula O1B, Formula O1C, Formula O2, Formula O3, Formula O4, Formula O4:K52, Formula O4:K6, Formula O5, Formula O5ab, Formula O5ac, Formula O6, Formula O6:K2; K13; K15, Formula O6:K54, Formula O7, Formula O8, Formula O9, Formula O10, Formula O11, Formula O12, Formula O13, Formula O14, Formula O15, Formula O16, Formula O17, Formula O18, Formula O18A, Formula O18ac, Formula O18A1, Formula O18B, Formula O18B1, Formula O19, Formula O20, Formula O21, Formula O22, Formula O23, Formula O23A, Formula O24, Formula O25, Formula O25a, Formula O25b, Formula O26, Formula O27, Formula O28, Formula O29, Formula O30, Formula O32, Formula O33, Formula O34, Formula O35, Formula O36, Formula O37, Formula O38, Formula O39, Formula O40, Formula O41, Formula O42, Formula O43, Formula O44, Formula O45, Formula O45, Formula O45rel, Formula O46, Formula O48, Formula O49, Formula O50, Formula O51, Formula O52, Formula O53, Formula O54, Formula O55, Formula O56, Formula O57, Formula O58, Formula O59, Formula O60, Formula O61, Formula O62, Formula 62D1, Formula O63, Formula O64, Formula O65, Formula O66, Formula O68, Formula O69, Formula O70, Formula O71, Formula O73, Formula O73, Formula O74, Formula O75, Formula O76, Formula O77, Formula O78, Formula O79, Formula O80, Formula O81, Formula O82, Formula O83, Formula O84, Formula O85, Formula O86, Formula O87, Formula O88, Formula O89, Formula O90, Formula O91, Formula O92, Formula O93, Formula O95, Formula O96, Formula O97, Formula O98, Formula O99, Formula O100, Formula O101, Formula O102, Formula O103, Formula O104, Formula O105, Formula O106, Formula O107, Formula O108, Formula O109, Formula O110, Formula O111, Formula O112, Formula O113, Formula O114, Formula O115, Formula O116, Formula O117, Formula O118, Formula O119, Formula O120, Formula O121, Formula O123, Formula O124, Formula O125, Formula O126, Formula O127, Formula O128, Formula O129, Formula O130, Formula O131, Formula O132, Formula O133, Formula O134, Formula O135, Formula O136, Formula O137, Formula O138, Formula O139, Formula O140, Formula O141, Formula O142, Formula O143, Formula O144, Formula O145, Formula O146, Formula O147, Formula O148, Formula O149, Formula O150, Formula O151, Formula O152, Formula O153, Formula O154, Formula O155, Formula O156, Formula O157, Formula O158, Formula O159, Formula O160, Formula O161, Formula O162, Formula O163, Formula O164, Formula O165, Formula O166, Formula O167, Formula O168, Formula O169, Formula O170, Formula O171, Formula O172, Formula O173, Formula O174, Formula O175, Formula O176, Formula O177, Formula O178, Formula O179, Formula O180, Formula O181, Formula O182, Formula O183, Formula O184, Formula O185, Formula O186, Formula O187. 
     
     
         12 . The composition according to  claim 11 , wherein the saccharide derived from  K. pneumoniae  is conjugated to a carrier protein; and the saccharide derived from  E. coli  is conjugated to a carrier protein. 
     
     
         13 . The composition according to  claim 11 , further comprising a polypeptide derived from  K. pneumoniae.    
     
     
         14 . A composition comprising at least one saccharide derived from any one  K. pneumoniae  type selected from the group consisting of O1, O2, O3, and O5; and at least one saccharide comprising a structure selected from any one of Formula O1, Formula O1A, Formula O1B, Formula O1C, Formula O2, Formula O3, Formula O4, Formula O4:K52, Formula O4:K6, Formula O5, Formula O5ab, Formula O5ac, Formula O6, Formula O6:K2; K13; K15, Formula O6:K54, Formula O7, Formula O8, Formula O9, Formula O10, Formula O11, Formula O12, Formula O13, Formula O14, Formula O15, Formula O16, Formula O17, Formula O18, Formula O18A, Formula O18ac, Formula O18A1, Formula O18B, Formula O18B1, Formula O19, Formula O20, Formula O21, Formula O22, Formula O23, Formula O23A, Formula O24, Formula O25, Formula O25a, Formula O25b, Formula O26, Formula O27, Formula O28, Formula O29, Formula O30, Formula O32, Formula O33, Formula O34, Formula O35, Formula O36, Formula O37, Formula O38, Formula O39, Formula O40, Formula O41, Formula O42, Formula O43, Formula O44, Formula O45, Formula O45, Formula O45rel, Formula O46, Formula O48, Formula O49, Formula O50, Formula O51, Formula O52, Formula O53, Formula O54, Formula O55, Formula O56, Formula O57, Formula O58, Formula O59, Formula O60, Formula O61, Formula O62, Formula 62D 1 , Formula O63, Formula O64, Formula O65, Formula O66, Formula O68, Formula O69, Formula O70, Formula O71, Formula O73, Formula O73, Formula O74, Formula O75, Formula O76, Formula O77, Formula O78, Formula O79, Formula O80, Formula O81, Formula O82, Formula O83, Formula O84, Formula O85, Formula O86, Formula O87, Formula O88, Formula O89, Formula O90, Formula O91, Formula O92, Formula O93, Formula O95, Formula O96, Formula O97, Formula O98, Formula O99, Formula O100, Formula O101, Formula O102, Formula O103, Formula O104, Formula O105, Formula O106, Formula O107, Formula O108, Formula O109, Formula O110, Formula O111, Formula O112, Formula O113, Formula O114, Formula O115, Formula O116, Formula O117, Formula O118, Formula O119, Formula O120, Formula O121, Formula O123, Formula O124, Formula O125, Formula O126, Formula O127, Formula O128, Formula O129, Formula O130, Formula O131, Formula O132, Formula O133, Formula O134, Formula O135, Formula O136, Formula O137, Formula O138, Formula O139, Formula O140, Formula O141, Formula O142, Formula O143, Formula O144, Formula O145, Formula O146, Formula O147, Formula O148, Formula O149, Formula O150, Formula O151, Formula O152, Formula O153, Formula O154, Formula O155, Formula O156, Formula O157, Formula O158, Formula O159, Formula O160, Formula O161, Formula O162, Formula O163, Formula O164, Formula O165, Formula O166, Formula O167, Formula O168, Formula O169, Formula O170, Formula O171, Formula O172, Formula O173, Formula O174, Formula O175, Formula O176, Formula O177, Formula O178, Formula O179, Formula O180, Formula O181, Formula O182, Formula O183, Formula O184, Formula O185, Formula O186, Formula O187. 
     
     
         15 . The composition according to  claim 14 , further comprising a polypeptide derived from FimH or a fragment thereof. 
     
     
         16 . The composition according to  claim 14 , wherein the  E. coli  saccharide comprises Formula O8. 
     
     
         17 . The composition according to  claim 14 , wherein the  E. coli  saccharide comprises Formula O9. 
     
     
         18 . The composition according to  claim 14 , further comprising a polypeptide derived from  K. pneumoniae.    
     
     
         19 . The composition according to any of  claims 1 - 18 , wherein the saccharide is covalently bound to a carrier protein. 
     
     
         20 . The composition according to  claim 19 , wherein the saccharide further comprises a 3-deoxy-d-manno-oct-2-ulosonic acid (KDO) moiety. 
     
     
         21 . The composition according to  claim 19 , wherein the carrier protein is selected from any one of CRM197, diphtheria toxin fragment B (DTFB), DTFB C8, Diphtheria toxoid (DT), tetanus toxoid (TT), fragment C of TT, pertussis toxoid, cholera toxoid, or exotoxin A from  Pseudomonas aeruginosa ; detoxified Exotoxin A of  P. aeruginosa  (EPA), maltose binding protein (MBP), detoxified hemolysin A of  S. aureus , clumping factor A, clumping factor B, Cholera toxin B subunit (CTB),  Streptococcus pneumoniae  Pneumolysin and detoxified variants thereof,  C. jejuni  AcrA,  C. jejuni  natural glycoproteins and Streptococcal C5a peptidase (SCP). 
     
     
         22 . A method of eliciting an immune response against  Escherichia coli  in a mammal, comprising administering to the mammal an effective amount of the composition according to any one of  claims 1 - 21 . 
     
     
         23 . The method according to  claim 22 , wherein the immune response comprises opsonophagocytic antibodies against  E. coli.    
     
     
         24 . The method according to  claim 22 , wherein the immune response protects the mammal from an  E. coli  infection. 
     
     
         25 . A method of eliciting an immune response against  Klebsiella pneumoniae  in a mammal, comprising administering to the mammal an effective amount of the composition according to any one of  claims 1 - 24 . 
     
     
         26 . The method according to  claim 25 , wherein the immune response comprises opsonophagocytic antibodies against  Klebsiella pneumoniae.    
     
     
         27 . The method according to  claim 25 , wherein the immune response protects the mammal from a  Klebsiella pneumoniae  infection.

Join the waitlist — get patent alerts

Track US2021268095A1 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.