Nanodiamond particles and related devices and methods

Abstract

Nanodiamond particles and related devices and methods, such as nanodiamond particles for the detection and/or quantification of analytes, are generally described. In some embodiments, the device comprises a plurality of nanodiamond particles and a species bound to the nanodiamond particles. In certain embodiments, the plurality of nanodiamond particles may be exposed to a sample suspected of containing an analyte. In some cases, the analyte may bind to the species such that the presence of the analyte in the sample may be detected. In some embodiments, the devices, systems, and methods described herein are useful for the detection of an analyte in a sample obtained from a subject for, for example, diagnostic purposes. In some cases, the systems, devices, and methods described herein may be useful for diagnosing, prevent, treating, and/or managing a disease or bodily condition. In an exemplary embodiment, such systems, devices, and methods described herein may be useful for detecting and/or quantifying the presence of a virus (e.g., ebola) in a subject and/or a sample obtained from the subject.

1 . A diagnostic agent, said agent comprising a fluorescent nanodiamond particle chemically bonded to a polypeptide, ligand, and/or polynucleotide, wherein the fluorescent nanodiamond particle has an average cross-sectional dimension of greater than or equal to 250 nanometers and less than or equal to 1000 nanometers. 
     
     
         2 - 4 . (canceled) 
     
     
         5 . The diagnostic agent of  claim 1 , wherein the fluorescent nanodiamond particle and the polypeptide, ligand, and/or polynucleotide are covalently bonded. 
     
     
         6 - 31 . (canceled) 
     
     
         32 . A diagnostic agent as in  claim 1 , wherein the polypeptide is a disintegrin. 
     
     
         33 . (canceled) 
     
     
         34 . A diagnostic agent for detection of thrombotic events in a human or non-human animal, said agent comprising a nanodiamond particle chemically bonded to a disintegrin. 
     
     
         35 - 36 . (canceled) 
     
     
         37 . The diagnostic agent of  claim 34 , wherein the nanodiamond particle and the disintegrin are covalently bonded. 
     
     
         38 - 46 . (canceled) 
     
     
         47 . A diagnostic agent as in  claim 34 , wherein the nanodiamond particle has an average cross-sectional dimension of greater than or equal to 250 nanometers and less than or equal to 1000 nanometers. 
     
     
         48 . A diagnostic agent as in  claim 1 , wherein the polypeptide is a protein, an antibody, and/or an antigen. 
     
     
         49 . A diagnostic agent as in  claim 32 , wherein the disintegrin is selected from the group consisting of albolabrin, applagin, barbourin, batroxostatin, bitistatin, obtustatin, schistatin, echistatin, elegantin, eristicophin, flavoridin, halysin, kistrin, mojastin, rubistatin, tergeminin, salmosin and triflavin. 
     
     
         50 . A diagnostic agent as in  claim 1 , wherein the polypeptide or polynucleotide is covalently bound to the plurality of fluorescent nanodiamond particles. 
     
     
         51 . A diagnostic agent as in  claim 1 , wherein the plurality of fluorescent nanodiamond particles comprise an atomistic-type defect. 
     
     
         52 . A diagnostic agent as in  claim 51 , wherein the atomistic-type defect is a nitrogen-vacancy defect, a nitrogen-vacancy-nitrogen defect, or an Si-vacancy defect. 
     
     
         53 . A diagnostic agent as in  claim 1 , wherein the plurality of fluorescent nanodiamond particles are intrinsically fluorescent. 
     
     
         54 . A diagnostic agent as in  claim 34 , wherein the nanodiamond particle is intrinsically fluorescent. 
     
     
         55 . A diagnostic agent as in  claim 34 , wherein the disintegrin is selected from the group consisting of albolabrin, applagin, barbourin, batroxostatin, bitistatin, obtustatin, schistatin, echistatin, elegantin, eristicophin, flavoridin, halysin, kistrin, mojastin, rubistatin, tergeminin, salmosin and triflavin. 
     
     
         56 . A diagnostic agent as in  claim 34 , wherein the disintegrin is covalently bound to the plurality of nanodiamond particles. 
     
     
         57 . A diagnostic agent as in  claim 34 , wherein the plurality of nanodiamond particles comprise an atomistic-type defect. 
     
     
         58 . A diagnostic agent as in  claim 57 , wherein the atomistic-type defect is a nitrogen-vacancy defect, a nitrogen-vacancy-nitrogen defect, or an Si-vacancy defect.