Micro chamber plate

Abstract

The present invention relates to a micro chamber plate, and more particularly, to a micro chamber plate having micro chamber holes formed using a flowable film. Thus, samples can be injected into the micro chamber holes in a smoother manner compared to when samples are injected using a vacuum and/or centrifugal force. In addition, bubbles and excess samples in the micro chamber holes can be efficiently discharged, making it possible to perform reaction and analysis in a more accurate and efficient manner.

What is claimed is: 
     
         1 . A method for manufacturing a micro chamber plate, the method comprising the steps of:
 (a) attaching a flowable film to a top side of a micro chamber body;   (b) blow molding to attach the flowable film to a lateral side of each micro chamber hole and, at the same time, to form the flowable film at a bottom side of the micro chamber hole, thereby forming micro chamber holes; and   (c) welding a micro chamber body covering part to the flowable film on the top side of the micro chamber body.   
     
     
         2 . The method of  claim 1 , further comprising a step (b) of injecting a specific component for analyzing biological substances into each micro chamber hole, between steps (b) and (c). 
     
     
         3 . The method of  claim 1 , wherein the blow molding in the step (b) is performed at a temperature of 100° C. to 300° C. 
     
     
         4 . The method of  claim 1 , wherein the micro chamber plate of  claim 1 , wherein in step (a), an adhesive film having an adhesive property is further provided between the flowable film and the micro chamber body or between the flowable film and the micro chamber body covering part. 
     
     
         5 . The method of  claim 1 , wherein the flowable film is transparent such that it allows optical measurement. 
     
     
         6 . The method of  claim 5 , wherein the flowable film is made of a material selected from among polyethylene, polyethylene terephthalate, stretched or non-stretched polypropylene, polycarbonate, PMMA, and a copolymer thereof. 
     
     
         7 . The method of  claim 1 , wherein the micro chamber body covering part has formed therein at least one sample inlet per micro chamber hole. 
     
     
         8 . The method of  claim 1 , wherein the micro chamber body covering part is made of a metal or a polymer film. 
     
     
         9 . The method of  claim 1 , wherein the micro chamber body covering part is made of a porous membrane having a pore size of 0.2-5.0 μm. 
     
     
         10 . The method of  claim 1 , wherein each sample inlet has an area of 0.05-60% based on total area of each micro chamber hole. 
     
     
         11 . The method of  claim 1 , wherein in step (c), the micro chamber body covering part is bonded to the flowable film by at least one method selected from thermal bonding, ultrasonic bonding, and adhesive agent coating. 
     
     
         12 . The method of  claim 1 , wherein the surface of the micro chamber body is coated with at least one material selected from a polyester-based polymer resin and a polyethylene-based hot-melt adhesive. 
     
     
         13 . The method of  claim 1 , further comprising steps of: (d) injecting the sample; and (e) sealing the sample inlet, after the step (c). 
     
     
         14 . The method of  claim 13 , wherein the sealing of the sample inlet is performed using at least one material selected from the group consisting of a polymer film, oil, silicone, paraffin and an adhesive agent.