Methods and systems for a digital pcr experiment designer

Abstract

A computer-implemented method for designing a digital PCR (dPCR) experiment is provided. The method includes receiving, from a user, a selection of optimization type. The optimization type may be maximizing the dynamic range, minimizing the number of substrates including reaction sites needed for the experiment, determining a dilution factor, or determining the lower limit of detection, for example. The method further includes receiving, from the user, a precision measure for an experiment, and a minimum concentration of a target in a reaction site for the experiment. The method also includes determining a set of dPCR experiment design factors for the experiment based on the optimization type. The set of dPCR experiment design factors is then displayed to the user.

What is claimed is: 
     
         1 . A method for designing a digital PCR (dPCR) experiment, the method comprising:
 receiving, from a user, a selection of optimization type;   receiving, from the user, a precision measure for an experiment;   receiving, from the user, a minimum concentration of a target in a reaction site for the experiment;   determining a set of dPCR experiment design factors for the experiment based on the optimization type; and   displaying the set of dPCR experiment design factors to the user.