Cell-derived particles presenting heterologous cd24 and use thereof in therapy

Abstract

A method of producing cell derived particles is disclosed. The method comprising isolating cell-derived particles from a biological sample comprising cells modified to present CD24 so as to obtain a preparation of the cell-derived particles substantially devoid of intact cells. Cell derived particles, a culture medium and a cell culture are also disclosed.

What is claimed is: 
     
         1 . A method of producing cell derived particles, the method comprising isolating cell-derived particles from a biological sample comprising cells modified to present CD24 so as to obtain a preparation of the cell-derived particles substantially devoid of intact cells. 
     
     
         2 . The method of  claim 1 , further comprising modifying cells to present CD24 to obtain said cells modified to present CD24 prior to said isolating cell-derived particles from a biological sample. 
     
     
         3 . The method of  claim 2 , further comprising culturing the cells modified to present CD24 prior to said isolating cell-derived particles from a biological sample. 
     
     
         4 . The method of  claim 1 , wherein the cells are cultured in a serum-free culture medium. 
     
     
         5 . The method of  claim 4 , wherein said culture medium comprises Expi medium. 
     
     
         6 . The method of  claim 1 , wherein the cells are cultured in a suspension culture. 
     
     
         7 . The method of  claim 6 , wherein the suspension culture is in the absence of insulin and albumin. 
     
     
         8 . The method of  claim 1 , wherein the cells are cultured in a 2D culture. 
     
     
         9 . The method of  claim 8 , wherein said 2D culture comprises insulin and albumin. 
     
     
         10 . The method of  claim 1 , wherein said preparation of the cell-derived particles comprises about 1×10 10 -1×10 15  cell derived particles per liter. 
     
     
         11 . The method of  claim 6 , wherein when said cells are cultured in a suspension culture, said preparation of the cell-derived particles comprises at least about 3 times more cell derived particles as compared to cells cultured in a 2D culture. 
     
     
         12 . A culture medium comprising Expi medium, insulin and albumin. 
     
     
         13 . A cell culture comprising cells and the medium of  claim 12 . 
     
     
         14 . Cell-derived particles presenting heterologous CD24 produced according to the method of  claim 1 . 
     
     
         15 . The method of  claim 1 , wherein said CD24 is as set forth in SEQ ID NO: 9 or encodable by SEQ ID NO: 8. 
     
     
         16 . The method of  claim 1 , wherein said cell-derived particles are selected from the group consisting of exosomes, ARMM, microvesicles, exomeres, membrane particles, membrane vesicles and ectosomes. 
     
     
         17 . The method of  claim 1 , wherein said cell-derived particles have a mean particle diameter of about 80 to about 220 nm. 
     
     
         18 . The method of  claim 1 , wherein said cell-derived particles are exosomes. 
     
     
         19 . The method of  claim 1 , wherein said cells are cells of an animal or a human tissue. 
     
     
         20 . The method of  claim 1 , wherein said cells are healthy cells. 
     
     
         21 . The method of  claim 1 , wherein said cells are genetically modified cells. 
     
     
         22 . The method of  claim 1 , wherein said cells are fibroblast cells or kidney cells. 
     
     
         23 . The method of  claim 1 , wherein said cells are HEK-293 cells.