Multisignal reagents for labeling analytes

Abstract

Provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.

What is claimed is: 
     
         1 . A method for providing an oligonucleotide labeled at each of the 5′ end and the 3′ end of the oligonucleotide, comprising the steps of:
 providing an DNA oligonucleotide labeled with a first member of a first binding pair at its 5′ end; 
 adding deoxynucleotides labeled with a first member of a second binding pair to the 3′ end of the oligonucleotide using terminal transferase. 
 
     
     
         2 . The method of  claim 1 , wherein the first member of the first binding pair and the first member of the second binding pair are the same. 
     
     
         3 . The method of  claim 1 , wherein the first member of the first binding pair and the first member of the second binding pair are different. 
     
     
         4 . The method of  claim 1 , wherein the first member of the first binding pair is streptavidin or avidin. 
     
     
         5 . The method of  claim 1 , therein the first member of the second binding pair is digoxigenin. 
     
     
         6 . The method of  claim 4 , wherein the first member of the second binding pair is digoxigenin.