Enhancing immunogenicity of streptococcus pneumoniae polysaccharide-protein conjugates

Abstract

The present invention provides immunogenic compositions having one or more polysaccharide-protein conjugates in which one or more polysaccharides from Streptococcus pneumoniae bacterial capsules are conjugated to a carrier protein in an aprotic solvent such as dimethylsulfoxide (DMSO). The present invention also provides methods for providing an enhanced immune response to a pneumococcal polysaccharide protein conjugate vaccine comprising administering to a human subject an immunogenic composition comprising polysaccharide-protein conjugates prepared in DMSO conditions.

1 . An immunogenic composition comprising polysaccharide from  S. pneumoniae  serotype conjugated to a carrier protein, wherein the conjugation reaction whereby the polysaccharide is conjugated to the carrier protein is in an aprotic solvent. 
     
     
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         7 . The immunogenic composition of  claim 1 , wherein the conjugation reaction is reductive amination. 
     
     
         8 . The immunogenic composition of  claim 1 , wherein the aprotic solvent is dimethylsulfoxide (DMSO). 
     
     
         9 . The immunogenic composition of  claim 1 , wherein the carrier protein is CRM 197 . 
     
     
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         54 . The immunogenic composition of  claim 1 , wherein the carrier protein is selected from the group consisting of Outer Membrane Protein Complex (OMPC), tetanus toxoid, diphtheria toxoid, protein D and CRM 197 . 
     
     
         55 . The immunogenic composition of  claim 1 , wherein the carrier protein is selected from the group consisting of tetanus toxoid, diphtheria toxoid, and CRM 197 .