Compositions and methods for preventing and treating oral diseases

Abstract

Methods for the prevention and treatment of oral cavity diseases caused by dental plaque/biofilm, such as dental caries, gingivitis and periodontitis, through anti-infective properties are disclosed. The methods use a combination of EDTA and citrate. The anti-infective properties of a composition include reduction or killing of anaerobic/aerobic/facultative gram-negative and gram-positive oral bacteria occurring in polymicrobial dental biofilms. The composition may be in the form of wash, rinse, soak, paste, gel, spray, or other suitable form.

We claim: 
     
         1 . A method of preventing or treating oral cavity diseases comprising oral use of the composition comprising:
 (a) ethylenediaminetetraacetic acid (EDTA) between about 250 mg/L and about 1000 mg/L of the composition; and   (b) a citrate selected from one or more of sodium citrate and potassium citrate, wherein the citrate is at a concentration between about 1600 mg/L and about 3200 mg/L.   
     
     
         2 . The method of  claim 1 , wherein the EDTA and sodium citrate are present at about 2 mg/ml and 3 mg/ml, respectively. 
     
     
         3 . The method of  claim 1 , wherein said composition further comprises (c) a zinc salt selected from the group consisting of zinc lactate, zinc gluconate, zinc citrate, and zinc chloride, and wherein said zinc salt is at a concentration of 25 mg/L to 100 mg/L. 
     
     
         4 . The method of  claim 1 , where the composition is prepared as one or more of a mouthwash, an oral rinse, a spray, an abrasive dentifrice gel, a dentifrice, a denture wash, a denture soak, a topical agent, a denture adhesive, a denture cement, a lozenge, and a pet chew biscuit. 
     
     
         5 . The method of  claim 4 , wherein the composition further comprises one or more ingredients selected from the group consisting of: water, a buffer, a stabilizing agent, a binding agent, a gelling agent, a desensitizing agent, a teeth whitening agent, an antiplaque deposition aide, a surfactant, a fluoride, a herbal, a vitamin, a mineral, an antimicrobial, an antibiotic, a pH adjuster, a flavor, and a color. 
     
     
         6 . The method of  claim 1 , wherein the EDTA is disodium EDTA. 
     
     
         7 . The method of  claim 1 , wherein the composition is prepared as one or more of a mouth-wash/rinse, a spray, solutions, a gel, a water additive, an abrasive/non-dentifrice gel, a paste, a denture wash, a denture soak, a denture adhesive or cement, a chewing gum, a candy, a lozenge, a biscuit and a soft drink. 
     
     
         8 . The method of  claim 1  wherein the composition comprises a liposomal or nanoparticle delivery system. 
     
     
         9 . The composition of  claim 1 , wherein the disodium EDTA is present at about 500 mg/L. 
     
     
         10 . The method as set forth in  claim 1  wherein the oral cavity disease is associated with dental biofilm. 
     
     
         11 . The method as claimed in  claim 10 , wherein the oral cavity disease is selected from one or more of, dental caries, gingivitis, periodontitis, oral bacterial infections and diseases. 
     
     
         12 . The method as set forth in  claim 1  further comprising multiple exposures to the composition. 
     
     
         13 . The method as claimed in  claim 1 , wherein the disorders of the oral cavity are pathological disorders associated with one or more of the dentition, implants and complications of surgery of the oral cavity. 
     
     
         14 . The method as claimed in  claim 1  wherein the method is used to treat one or more of humans, domestic and farm animals, and zoo, sports or pet animals, such as dogs, horses, cats, cattle, pigs and sheep. 
     
     
         15 . The method as claimed in  claim 14 , wherein the method is used to treat humans, dogs, cats or horses. 
     
     
         16 . The method as claimed in  claim 14 , wherein the method is used to treat humans. 
     
     
         17 . The method as claimed in  claim 14 , wherein the method is used to treat dogs. 
     
     
         18 . The method as claimed in  claim 1 , wherein the method is used to treat oral diseases associated with bacteria. 
     
     
         19 . The method as claimed in  claim 18 , wherein the bacteria is selected from the group consisting of  Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguis  ( sanguinis ),  Streptococcus gordonii, Streptococcus oralis, Streptococcus mitis, Actinomyces odontolyticus, Actinomyces viscosus, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Bacteroides forsythus, Treponema denticola, Fusobacterium nucleatum, Campylobacter rectus, Eikenella corrodens, Veillonella  spp.,  Micromonas micros, Porphyromonas cangingivalis, Haemophilus actinomycetemcomitans Actinomyces  spp.,  Bacillus  spp.,  Mycobacterium  spp.,  Fusobacterium  spp.,  Streptococcus  spp.,  Staphylococcus aureus, Streptococcus pyogenes, Streptococcus agalectiae, Proteus mirabilis, Klebsiella pneumoniae, E. coli, Acinetobacter  spp.,  Enterococcus  spp.,  Prevotella  spp.,  Porphyromonas  spp.,  Clostridium  spp.,  Stenotrophomonas maltophilia, P. cangingivalis , and  Candida albicans.