Production Of Fatty Acid Derivatives

Abstract

The disclosure relates to recombinant host cells including strain modifications effective to improve titer, yield and/or productivity of fatty acid derivatives. The disclosure further relates to cell cultures including the recombinant host cells for the fermentative production of fatty acid derivatives and compositions thereof.

1 .- 26 . (canceled) 
     
     
         27 . A recombinant host cell comprising a genetically engineered polynucleotide sequence encoding a phosphoenolpyruvate carboxylase (ppc) polypeptide, wherein said recombinant host cell produces a fatty acid derivative composition at a higher titer, yield or productivity than a corresponding wild type host cell when cultured in a medium containing a carbon source under conditions effective to express said ppc polypeptide. 
     
     
         28 . A cell culture comprising the recombinant host cell according to  claim 27 . 
     
     
         29 . The cell culture of  claim 28 , wherein said medium comprises a fatty acid derivative composition. 
     
     
         30 . The cell culture of  claim 29 , wherein the fatty acid derivative composition comprises at least one fatty acid derivative selected from the group consisting of fatty acid, a fatty ester, a fatty alcohol, a fatty aldehyde, an alkane, a terminal olefin, an internal olefin, and a ketone. 
     
     
         31 . The cell culture of  claim 28 , wherein the fatty acid derivative is a C6, C8, C10, C12, C13, C14, C15, C16, C17, or C18 fatty acid derivative. 
     
     
         32 . The cell culture of  claim 28 , wherein the fatty acid derivative is a C10:1, C12:1, C14:1, C16:1, or C18:1 unsaturated fatty acid derivative. 
     
     
         33 . The cell culture of  claim 29 , wherein the fatty acid derivative composition comprises one or more of C8, C10, C12, C14, C16, and C18 fatty acid derivatives. 
     
     
         34 . The cell culture of  claim 29 , wherein the fatty acid derivative composition comprises fatty acids. 
     
     
         35 .- 41 . (canceled) 
     
     
         42 . The cell culture of  claim 29 , wherein the fatty acid derivative composition comprises fatty acid derivatives having a double bond at position 7 in the carbon chain between C7 and C8 from the reduced end of the fatty alcohol. 
     
     
         43 . The cell culture of  claim 29 , wherein the fatty acid derivative composition comprises unsaturated fatty acid derivatives. 
     
     
         44 . The cell culture of  claim 29 , wherein the fatty acid derivative composition comprises saturated fatty acid derivatives. 
     
     
         45 . The cell culture of  claim 29 , wherein the fatty acid derivative composition comprises branched chain fatty acid derivatives. 
     
     
         46 . The cell culture of  claim 28 , wherein said recombinant host cell has a titer that is at least about 5% greater than a titer of said corresponding wild type host cell when cultured under the same conditions as the recombinant host cell. 
     
     
         47 . The cell culture of  claim 46 , wherein said recombinant host cell has a titer of from about 1 g/L to about 250 g/L. 
     
     
         48 . The cell culture of  claim 47 , wherein said recombinant host cell has a titer of from about 90 g/L to about 120 g/L. 
     
     
         49 . The cell culture of  claim 28 , wherein said recombinant host cell has a yield that is at least about 10% to about 40%. 
     
     
         50 . The cell culture of  claim 49 , wherein said recombinant host cell has a yield of about 25%. 
     
     
         51 . The cell culture of  claim 28 , wherein said productivity ranges from about 0.7 mg/L/hr to about 3 g/L/hr.