Methods and materials for treating cancer

Abstract

This document provides methods and materials related to treating cancer. For example, methods and materials for using nucleic acid libraries to treat cancer are provided.

What is claimed is: 
     
         1 . A method for treating cancer present in a mammal, wherein said method comprises administering, to said mammal, a nucleic acid library under conditions wherein nucleic acid members of said nucleic acid library are expressed in said mammal, thereby reducing the number of viable cancer cells present within said mammal. 
     
     
         2 . The method of  claim 1 , wherein said mammal is a human. 
     
     
         3 . The method of  claim 1 , wherein said administration is an intratumoral or intravenous administration. 
     
     
         4 . The method of  claim 1 , wherein said nucleic acid library is a cDNA library. 
     
     
         5 . The method of  claim 1 , wherein said nucleic acid library is administered to said mammal using a virus. 
     
     
         6 . The method of  claim 5 , wherein said virus is an oncolytic virus. 
     
     
         7 . The method of  claim 5 , wherein said virus is a vesicular stomatitis virus. 
     
     
         8 . The method of  claim 1 , wherein said nucleic acid members are cDNA molecules, wherein each cDNA molecule encodes a polypeptide. 
     
     
         9 . The method of  claim 1 , wherein said nucleic acid library comprises a plurality of different nucleic acid members, and wherein each of said plurality of different nucleic acid members encodes a different mammalian polypeptide. 
     
     
         10 . The method of  claim 9 , wherein the different mammalian polypeptides are polypeptides of a member of the same species as said mammal. 
     
     
         11 . The method of  claim 9 , wherein the different mammalian polypeptides are polypeptides of a member of a different species than the species of said mammal. 
     
     
         12 . The method of  claim 9 , wherein the different mammalian polypeptides are polypeptides of said mammal. 
     
     
         13 . The method of  claim 9 , wherein the different mammalian polypeptides are polypeptides of said cancer. 
     
     
         14 . The method of  claim 9 , wherein the different mammalian polypeptides are polypeptides of an organ or tissue that is an organ or tissue of origin of said cancer. 
     
     
         15 . The method of  claim 14 , wherein said cancer is prostrate cancer, and said different mammalian polypeptides are polypeptides expressed by prostate cells. 
     
     
         16 . The method of  claim 14 , wherein said cancer is lung cancer, and said different mammalian polypeptides are polypeptides expressed by lung cells. 
     
     
         17 . The method of  claim 14 , wherein said cancer is skin cancer, and said different mammalian polypeptides are polypeptides expressed by skin cells. 
     
     
         18 . The method of  claim 1 , wherein said nucleic acid library comprises a collection of greater than 10 4  different nucleic acid members, and wherein each of said 10 4  different nucleic acid members encodes a different mammalian polypeptide. 
     
     
         19 . The method of  claim 1 , wherein said nucleic acid library comprises a collection of greater than 10 5  different nucleic acid members, and wherein each of said 10 5  different nucleic acid members encodes a different mammalian polypeptide. 
     
     
         20 . The method of  claim 1 , wherein said nucleic acid library comprises a collection of greater than 10 6  different nucleic acid members, and wherein each of said 10 6  different nucleic acid members encodes a different mammalian polypeptide. 
     
     
         21 . The method of  claim 1 , wherein said nucleic acid library comprises a collection of greater than 10 7  different nucleic acid members, and wherein each of said 10 7  different nucleic acid members encodes a different mammalian polypeptide. 
     
     
         22 . The method of  claim 1 , wherein said nucleic acid library is administered to said mammal in combination with an adjuvant. 
     
     
         23 . The method of  claim 1 , wherein said adjuvant is selected from the group consisting of an HSP70 polypeptide, a vesicular stomatitis virus, BCG, a vaccinia virus, and an adenovirus. 
     
     
         24 . A composition for treating cancer present in a mammal, wherein said composition comprises a nucleic acid library comprising a plurality of different nucleic acid members, wherein each of said plurality of different nucleic acid members encodes a different mammalian polypeptide and is located within the genome of an oncolytic virus having the ability to infect a cell present within said mammal, and wherein said mammalian polypeptides are expressed within said mammal following administration of said composition to said mammal. 
     
     
         25 . The composition of  claim 24 , wherein said mammal is a human. 
     
     
         26 . The composition of  claim 24 , wherein said administration is an intratumoral or intravenous administration. 
     
     
         27 . The composition of  claim 24 , wherein said nucleic acid library is a cDNA library. 
     
     
         28 . The composition of  claim 24 , wherein said oncolytic virus is a vesicular stomatitis virus. 
     
     
         29 . The composition of  claim 24 , wherein said different mammalian polypeptides are polypeptides of a member of the same species as said mammal. 
     
     
         30 . The composition of  claim 24 , wherein said different mammalian polypeptides are polypeptides of a member of a different species than the species of said mammal. 
     
     
         31 . The composition of  claim 24 , wherein said different mammalian polypeptides are polypeptides of said mammal. 
     
     
         32 . The composition of  claim 24 , wherein said different mammalian polypeptides are polypeptides of said cancer. 
     
     
         33 . The composition of  claim 24 , wherein said different mammalian polypeptides are polypeptides of an organ or tissue that is an organ or tissue of origin of said cancer. 
     
     
         34 . The composition of  claim 24 , wherein said cancer is prostrate cancer, and said different mammalian polypeptides are polypeptides expressed by prostate cells. 
     
     
         35 . The composition of  claim 24 , wherein said cancer is lung cancer, and said different mammalian polypeptides are polypeptides expressed by lung cells. 
     
     
         36 . The composition of  claim 24 , wherein said cancer is skin cancer, and said different mammalian polypeptides are polypeptides expressed by skin cells. 
     
     
         37 . The composition of  claim 24 , wherein said nucleic acid library comprises a collection of greater than 10 4  different nucleic acid members, and wherein each of said 10 4  different nucleic acid members encodes a different mammalian polypeptide. 
     
     
         38 . The composition of  claim 24 , wherein said nucleic acid library comprises a collection of greater than 10 5  different nucleic acid members, and wherein each of said 10 5  different nucleic acid members encodes a different mammalian polypeptide. 
     
     
         39 . The composition of  claim 24 , wherein said nucleic acid library comprises a collection of greater than 10 6  different nucleic acid members, and wherein each of said 10 6  different nucleic acid members encodes a different mammalian polypeptide. 
     
     
         40 . The composition of  claim 24 , wherein said nucleic acid library comprises a collection of greater than 10 5  different nucleic acid members, and wherein each of said 10 7  different nucleic acid members encodes a different mammalian polypeptide. 
     
     
         41 . The composition of  claim 24 , wherein said composition lacks serum.