Dermal layer for grafting having improved graft survival rate and method for producing same

Abstract

The present invention relates to a dermal layer which is for grafting and has an improved graft survival rate, and a method for producing the same, wherein the dermal layer for grafting can be produced by filling a filling solution, including a DNA fragment mixture and chitosan, into an acellular dermal matrix from which cells have been removed. It was observed that the dermal layer for grafting produced in this manner, due to the filling solution filled therein and including a DNA fragment mixture and chitosan, increases the rate at which cells flow in from the tissue surrounding the graft and are fixed, and thereby alleviates an initial inflammatory reaction and promotes blending with the surrounding tissue.

1 . A dermal layer for grafting, produced by applying a loading solution comprising a DNA fragment mixture and a chitosan on to an acellular dermal matrix,
 wherein the DNA fragment mixture is from fish testis or sperm,   wherein the DNA fragment mixture and chitosan is mixed in a weight ratio of 20:1-10,000:1, and   wherein the dermal layer has an improved graft survival rate.   
     
     
         2 . The dermal layer for grafting of  claim 1 , wherein the fish is of the family Salmonidae. 
     
     
         3 . The dermal layer for grafting of  claim 1 , wherein the DNA fragment mixture has a molecular weight of 50 kDa to 10,000 kDa. 
     
     
         4 . The dermal layer for grafting of  claim 1 , wherein the chitosan has a molecular weight of 3 kDa to 1,000 kDa. 
     
     
         5 . The dermal layer for grafting of  claim 1 , wherein the DNA fragment mixture is in an amount of 0.01% by weight to 3% by weight, based on the total weight of the loading solution. 
     
     
         6 . The dermal layer for grafting of  claim 1 , wherein the chitosan is in an amount of 1×10 −6 % by weight to 0.15% by weight, based on the total weight of the loading solution. 
     
     
         7 . The dermal layer for grafting of  claim 1 , wherein the acellular dermal matrix is a biologically inactivated collagen-based tissue supplement material that has been obtained by removing cells and impurities from a dermal matrix and subjecting the dermal matrix to virus inactivation and then to lyophilization. 
     
     
         8 . The dermal layer for grafting of  claim 1 , wherein the dermal matrix is an autograft, allograft, or heterograft. 
     
     
         9 . An agent for treating skin injury, comprising the dermal layer for grafting of  claim 1 . 
     
     
         10 . The agent of  claim 9 , wherein the skin injury is caused by at least one selected from the group consisting of abrasion, incision, laceration, skin avulsion, skin bruise, puncture wound, and surgical skin excision.