Compositions and methods for preventing, slowing, and reversing skin aging

Abstract

Disclosed are methods of treating aging effects on a subject skin, comprising contacting the skin with a skin microbiome complex, including topical compositions containing YM Microbiome Complex and Advanced Probiotic Complex, methods of treatment using the compositions and methods of preparing the compositions for preventing, slowing, and reversing skin aging. Also disclosed are related formulations and methods of skin microbiome transplantation.

What is claimed is: 
     
         1 - 23 . (canceled) 
     
     
         24 . A skin microbiome complex comprising a sterilized supernatant from a culture of microbiome bacteria that has been collected from a surface of wrinkle-free skin of a human subject. 
     
     
         25 . The skin microbiome complex of  claim 24 , wherein the human subject is 18-28 years old. 
     
     
         26 . The skin microbiome complex of  claim 24 , wherein the microbiome bacteria was collected with a swab of the area of the cheek beside the nostril. 
     
     
         27 . The skin microbiome complex of  claim 24 , wherein the microbiome bacteria is an  S. epidermidis.    
     
     
         28 . The skin microbiome complex of  claim 24 , wherein the culture of microbiome bacteria was cultured anaerobically. 
     
     
         29 . The skin microbiome complex of  claim 28 , wherein the microbiome bacteria was incubated overnight in tryptic soy broth (TSB) at 37° C. 
     
     
         30 . The skin microbiome complex of  claim 28 , wherein the culture comprises a carbohydrate that is fermented when cultured anaerobically. 
     
     
         31 . The skin microbiome complex of  claim 30 , wherein the carbohydrate is selected from the group consisting of at least one of glycerol, sucrose, β-lactose, D-(+)-maltose and galactose. 
     
     
         32 . The skin microbiome complex of  claim 24 , wherein the skin microbiome complex can increase ceramide levels when incubated with human keratinocytes. 
     
     
         33 . The skin microbiome complex of  claim 24 , wherein the skin microbiome complex is formulated in a liquid, gel, lotion, or cream. 
     
     
         34 . The skin microbiome complex of  claim 24 , further comprising aloe vera powder. 
     
     
         35 . The skin microbiome complex of  claim 24 , further comprising HE Cellulose. 
     
     
         36 . The skin microbiome complex of  claim 24 , further comprising one or more anti-aging compounds selected from the group consisting of retinol, epidermal growth factor, alpha hydroxy acid, a beta hydroxy acid, an exfoliant, argireline, a copper peptide, coenzyme Q10, an anti-oxidant, niacinamide, hyaluronic acid, polylactic acid,  Boswellia serrata,  and a sunscreen. 
     
     
         37 . The skin microbiome complex of  claim 24 , further comprising one or more anti-oxidants or brightening agents. 
     
     
         38 . A method of treating aging effects on skin of a human subject, the method comprising administering to the subject the skin microbiome complex of  claim 24 . 
     
     
         39 . The method of  claim 38 , wherein the skin microbiome complex is contacted with the skin twice a day for at least 7 days, wherein the human notices improvement in their skin appearance after the 7 days. 
     
     
         40 . The method of  claim 38 , wherein the skin microbiome complex is contacted with the skin twice a day for at least 15 days. 
     
     
         41 . The method of  claim 38 , wherein the human subject is 50 years old or older. 
     
     
         42 . The method of  claim 38 , further comprising,
 a. washing to skin with a mild cleanser in the morning and evening;   b. applying the anti-aging preparation containing the skin microbiome complex in the morning and evening;   c. taking anti-aging probiotics once a day; and   d. repeating (a)-(c) daily.   
     
     
         43 . The method of  claim 42 , further comprising the addition of zinc, another supplement or a vitamin to any of (a)-(d).