Polypeptides having rnase activity

Abstract

The present invention relates to polypeptides having RNase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

1 . A polypeptide of the clades EYTV, EAD or YPH having RNase activity, selected from the group consisting of:
 (a) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 3;   (b) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 6;   (c) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 9;   (d) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 12;   (e) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 15;   (f) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 57;   (g) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 58;   (h) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 59;   (i) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 60;   (j) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 61;   (k) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 62;   (l) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 63;   (m) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 64;   (n) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 65;   (o) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 66;   (p) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 67;   (q) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 72;   (r) a polypeptide having at least 80% sequence identity to the polypeptide of SEQ ID NO: 73;   (s) a variant of the polypeptide selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 12, SEQ ID NO: 15, SEQ ID NO: 57, SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 60, SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 63, SEQ ID NO: 64, SEQ ID NO: 65, SEQ ID NO: 66, SEQ ID NO: 67, SEQ ID NO: 72 and SEQ ID NO: 73, wherein the variant has RNase activity and comprises one or more amino acid substitutions, and/or one or more amino acid deletions, and/or one or more amino acid insertions or any combination thereof in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20 positions;   (t) a polypeptide comprising the polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), (l), (m), (n), (o), (p), (q), (r) or (s) and an N-terminal and/or C-terminal His-tag and/or HQ-tag;   (u) a polypeptide comprising the polypeptide of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), (l), (m), (n), (o), (p), (q), (r) or (s) and an N-terminal and/or C-terminal extension of between 1 and 10 amino acids;   (v) a fragment of the polypeptide of (a), (b), (c), (d), ((e), (f), (g), (h), (i), (j), (k), (l), (m), (n), (o), (p), (q), (r) or (s) having RNase activity and having at least 90% of the length of the mature polypeptide;   (w) a polypeptide comprising one or more of the motif(s) EYTV (SEQ ID NO: 28), [YRF]E[AYFWC]D (SEQ ID NO: 29), IGGD (SEQ ID NO: 30), YPH (SEQ ID NO: 31), HTGA (SEQ ID NO: 32) or DRV (SEQ ID NO:33); and   (x) a polypeptide comprising one or more of the motifs YXEYTVXTPXXXXRGXRR (SEQ ID NO: 78), [WY][YRF]E[AYFWC]D[IV] (SEQ ID NO: 79), GXXIGGDXFXN (SEQ ID NO: 80), YPHX[YFA]X[ND]XE (SEQ ID NO: 81), PGXDRV (SEQ ID NO: 82) or THTGA[SR]G (SEQ ID NO: 83).   
     
     
         2 - 15 . (canceled) 
     
     
         16 . A composition comprising a surfactant and a polypeptide of the clade EYTV, EAD or YPH having RNase activity, wherein the polypeptide is selected from the group consisting of:
 (a) a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO: 57;   (b) a polypeptide comprising the polypeptide of (a) and an N-terminal and/or C-terminal His-tag and/or HQ-tag;   (c) a polypeptide comprising the polypeptide of (a) and an N-terminal and/or C-terminal extension of between 1 and 10 amino acids; and   (d) a fragment of the polypeptide of SEQ ID NO: 57 having RNase activity and at least 90% of the length of SEQ ID NO: 57.   
     
     
         17 . The composition of  claim 16 , wherein the polypeptide has at least 96% sequence identity to the polypeptide of SEQ ID NO: 57. 
     
     
         18 . The composition of  claim 16 , wherein the polypeptide has at least 97% sequence identity to the polypeptide of SEQ ID NO: 57. 
     
     
         19 . The composition of  claim 16 , wherein the polypeptide has at least 98% sequence identity to the polypeptide of SEQ ID NO: 57. 
     
     
         20 . The composition of  claim 16 , wherein the polypeptide has at least 99% sequence identity to the polypeptide of SEQ ID NO: 57. 
     
     
         21 . The composition of  claim 16 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 57. 
     
     
         22 . The composition of  claim 16 , wherein the polypeptide consists of the amino acid sequence of SEQ ID NO: 57. 
     
     
         23 . The composition of  claim 16 , wherein the polypeptide is a fragment of the polypeptide of SEQ ID NO: 57 having RNase activity and at least 90% of the length of SEQ ID NO: 57. 
     
     
         23 . The composition of  claim 16 , which is in the form of a bar, a homogenous tablet, a tablet having two or more layers, a pouch having one or more compartments, a regular or compact powder, a granule, a paste, a gel, or a regular, compact or concentrated liquid. 
     
     
         24 . The composition of  claim 16 , further comprising one or more detergent adjunct ingredient selected from the group consisting of builders, flocculating aids, chelating agents, dye transfer inhibitors, enzymes, enzyme stabilizers, enzyme inhibitors, catalytic materials, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, polymeric agents, clay soil removal/anti-redeposition agents, brighteners, suds suppressors, dyes, perfumes, structure elasticizing agents, fabric softeners, carriers, hydrotropes, builders and co-builders, fabric huing agents, anti-foaming agents, dispersants, processing aids, and pigments. 
     
     
         25 . The composition of  claim 16 , further comprising one or more enzymes selected from the group consisting of amylases, catalases, cellulases, cutinases, haloperoxygenases, lipases, mannanases, pectinases, pectin lyases, peroxidases, proteases, xanthanases, xyloglucanases, and mixtures thereof. 
     
     
         26 . A method for laundering a textile comprising:
 a. exposing the textile to a wash liquor comprising the composition of  claim 16 ; and   b. completing at least one wash cycle.   
     
     
         27 . The method of  claim 26 , further comprising rinsing the textile.