Maize event dp-004114-3 and methods for detection thereof

Abstract

The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

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         5 . A corn plant comprising the genotype of the corn event DP-004114-3, wherein said genotype comprises the nucleotide sequence set forth in SEQ ID NO: 27, and SEQ ID NO: 28. 
     
     
         6 . The corn plant of  claim 5 , wherein said genotype comprises the nucleotide sequence set forth in SEQ ID NO: 27. 
     
     
         7 . The corn plant of  claim 5 , wherein said genotype comprises the nucleotide sequence set forth in SEQ ID NO: 28. 
     
     
         8 . A corn event DP-004114-3, wherein a representative sample of seed of said corn event has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506. 
     
     
         9 . Plant parts of the corn event of  claim 8 . 
     
     
         10 . Seed comprising corn event DP-004114-3, wherein said seed comprises a DNA molecule selected from the group consisting of SEQ ID NO: 27 and SEQ ID NO: 28, wherein a representative sample of corn event DP-004114-3 seed of has been deposited with American Type Culture Collection (ATCC) with Accession No. PTA-11506. 
     
     
         11 . A corn plant, or part thereof, grown from the seed of  claim 10 . 
     
     
         12 . A transgenic seed produced from the corn plant of  claim 11  comprising event DP-004114-3. 
     
     
         13 . A transgenic corn plant, or part thereof, grown from the seed of  claim 12 . 
     
     
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         16 . A biological sample derived from the corn event DP-004114-3 of  claim 8 . 
     
     
         17 . The biological sample of  claim 16 , wherein said biological sample comprise plant, tissue, or seed of transgenic corn event DP-004114-3. 
     
     
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         19 . The biological sample of  claim 17 , wherein said biological sample is selected from the group consisting of corn flour, corn meal, corn syrup, corn oil, corn starch, and cereals manufactured in whole or in part to contain corn by-products. 
     
     
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         30 . A method for producing a corn plant resistant to at least corn rootworm, said method comprising:
 (a) sexually crossing a first parent corn plant with a second parent corn plant, wherein said first or second parent corn plant is the corn event of  claim 8 , thereby producing a plurality of first generation progeny plants;   (b) selecting a first generation progeny plant that is resistant to at least corn rootworm infestation;   (c) backcrossing the first generation progeny plant of step (b) with the parent plant that lacks corn event DP-004114-3 DNA, thereby producing a plurality of backcross progeny plants; and   (d) selecting from the backcross progeny plants, a plant that is resistant to at least corn rootworm infestation;   wherein the selected backcross progeny plant of step (d) comprises SEQ ID NO: 6.   
     
     
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