Artificial intelligence designed antimicrobial peptides

Abstract

De novo, artificial intelligence (AI) designed antimicrobial peptides (AMPs), antibacterial products comprising the AMPs and methods for treating bacterial infections using the products are provided. In one or more embodiments, the AMPs were designed using conditional latent attribute space sampling (CLaSS). The AMPs comprise up to twenty natural amino acids in length, including one with twelve and another with thirteen natural amino acids in length. The AMPs demonstrate low-toxicity and show high antimicrobial potency against diverse pathogens including multi-medication-resistant Gram negative Klebsiella pneumoniae.

What is claimed is: 
     
         1 . A synthetic peptide comprising twelve, natural amino acids in length and the amino acid sequence HILRMRIRQMMT (SEQ ID NO: 3), wherein the synthetic peptide has antimicrobial activity. 
     
     
         2 . The synthetic peptide of  claim 1 , further comprising a terminal amide with formula CONH2, resulting in the synthetic peptide having the sequence HILRMRIRQMMT-CONH2 (SEQ ID NO: 3 —CONH2). 
     
     
         3 . The synthetic peptide of  claim 1 , wherein the synthetic peptide has the antimicrobial activity against Gram negative bacteria and Gram positive bacteria. 
     
     
         4 . The synthetic peptide of  claim 1 , wherein the synthetic peptide has the antimicrobial activity against multi-medication resistant  Klebsiella pneumonia.    
     
     
         5 . The synthetic peptide of  claim 1 , wherein the synthetic peptide has the antimicrobial activity against bacteria selected from the group consisting of:  Pseudomonas aeruginosa, Acinetobacter baummannii, Staphylococcus aureus , and  Escherichia coli.    
     
     
         6 . The synthetic peptide of  claim 1 , wherein a minimum inhibitory concentration value of the synthetic peptide with regards to bacteria is less than a median lethal dose value of the synthetic peptide. 
     
     
         7 . The synthetic peptide of  claim 1 , wherein the synthetic peptide exhibits low toxicity as measured by a 50% lethal dose (LD 50 ) concentration in mice of 182.0 milligrams per kilogram mg/kg. 
     
     
         8 . The synthetic peptide of  claim 1 , wherein the synthetic peptide was designed using one or more artificial intelligence techniques. 
     
     
         9 . A synthetic peptide comprising thirteen, natural amino acids in length and the amino acid sequence ILLHAILGVRKKL (SEQ ID NO: 4), wherein the synthetic peptide has antimicrobial activity. 
     
     
         10 . The synthetic peptide of  claim 9 , further comprising a terminal amide with formula CONH2, resulting in the synthetic peptide having the sequence ILLHAILGVRKKL-CONH2 (SEQ ID NO: 4-CONH2). 
     
     
         11 . The synthetic peptide of  claim 9 , wherein the synthetic peptide has the antimicrobial activity against Gram negative bacteria and Gram positive bacteria. 
     
     
         12 . The synthetic peptide of  claim 9 , wherein the synthetic peptide has the antimicrobial activity against multi-medication resistant  Klebsiella pneumonia.    
     
     
         13 . The synthetic peptide of  claim 9 , wherein the synthetic peptide has the antimicrobial activity against bacteria selected from the group consisting of:  Pseudomonas aeruginosa, Acinetobacter baummannii, Staphylococcus aureus , and  Escherichia coli.    
     
     
         14 . The synthetic peptide of  claim 9 , wherein a minimum inhibitory concentration value of the synthetic peptide with regards to bacteria is less than a median lethal dose value of the synthetic peptide. 
     
     
         15 . The synthetic peptide of  claim 9 , wherein the synthetic peptide exhibits low toxicity as measured by a 50% lethal dose (LD 50 ) concentration in mice of 182.0 milligrams per kilogram mg/kg. 
     
     
         16 . The synthetic peptide of  claim 1 , wherein the synthetic peptide was designed using one or more artificial intelligence techniques. 
     
     
         17 . A synthetic peptide comprising thirteen, natural amino acids in length and the amino acid sequence YRAAMLRRQYMMT (SEQ ID NO: 5), wherein the synthetic peptide has antimicrobial activity. 
     
     
         18 . The synthetic peptide of  claim 17 , further comprising a terminal amide with formula CONH2, resulting in the synthetic peptide having the sequence YRAAMLRRQYMMT —CONH2 (SEQ ID NO: 5-CONH2). 
     
     
         19 . The synthetic peptide of  claim 17 , wherein the synthetic peptide has the antimicrobial activity against Gram negative bacteria and Gram positive bacteria. 
     
     
         20 . The synthetic peptide of  claim 17 , wherein a minimum inhibitory concentration value of the synthetic peptide with regards to bacteria is less than a median lethal dose value of the synthetic peptide.