Microbial compositions comprising ellagitannin and methods of use

Abstract

A combination is provided comprising an ellagitannin composition, and an enzymatic composition comprising one or more ellagitannin enzymes, where the one or more ellagitannin enzymes comprise a tannin acyl hydrolase enzyme, a gallate decarboxylase enzyme, or a combination thereof. Methods of making and using combinations disclosed herein are also provided.

1 .- 26 . (canceled) 
     
     
         27 . A method comprising administering to a subject:
 (i) an ellagitannin composition,   (ii) an enzymatic composition comprising an ellagitannin-enzyme-synthesizing (EES) microbe or an extract thereof, or   (iii) a combination thereof,    so that the subject is receiving:   (a) the ellagitannin composition, and   (b) the enzymatic composition comprising the EES microbe or an extract thereof; and    wherein the EES microbe is  Lactobacillus plantarum  MBT501.   
     
     
         28 . The method of  claim 27 , wherein the one or more ellagitannin enzymes comprises a tannin acyl hydrolase enzyme. 
     
     
         29 . The method of  claim 28 , wherein the tannin acyl hydrolase enzyme is a tanB tannase enzyme. 
     
     
         30 . The method of  claim 27 , wherein the one or more ellagitannin enzymes comprises a gallate decarboxylase enzyme. 
     
     
         31 . The method of  claim 30 , wherein the gallate decarboxylase enzyme is an lpdB gallate decarboxylase enzyme. 
     
     
         32 . The method of  claim 30 , wherein the gallate decarboxylase enzyme is an lpdC gallate decarboxylase enzyme. 
     
     
         33 . The method of  claim 27 , wherein the one or more ellagitannin enzymes comprises an lpdB gallate decarboxylase enzyme and an lpdC gallate decarboxylase enzyme. 
     
     
         34 . The method of  claim 27 , wherein the one or more ellagitannin enzymes comprises a tanB tannase enzyme, an lpdB gallate decarboxylase enzyme, and an lpdC gallate decarboxylase enzyme. 
     
     
         35 . The method of  claim 27 , wherein the EES microbe is found in nature. 
     
     
         36 . The method of  claim 27 , wherein the EES microbe is viable or alive. 
     
     
         37 . The method of  claim 36 , wherein the enzymatic composition includes a sufficient amount of the EES microbe to colonize the microbiome of a subject. 
     
     
         38 . The method of  claim 27 , wherein the EES microbe is lyophilized. 
     
     
         39 . The method of  claim 27 , wherein the ellagitannin composition is selected from the group consisting of a plant extract of pomegranate, strawberry, raspberry, cranberry, blackberry, cloudberry, artic blackberry, muscadine grapes, guava, a Myrtaceae family fruit, walnut, pecan, chestnut, cashew, almond, pistachio, hazelnut, brazil nut, macadamia red wine aging in oak barrels, muscadine grapes juice, pomegranate juice, tea, cognac, Indian gooseberry, beefsteak fungus, and combinations thereof. 
     
     
         40 . The method of  claim 27 , comprising administering a prebiotic. 
     
     
         41 . The method of  claim 40 , wherein the prebiotic is or comprises a fructooligosaccharide, an inulin, an isomaltooligosaccharide, a lactilol, a lactosucrose, a lactulose, a soy oligosaccharide, a transgalactooligosaccharide, a xylooligosaccharide, or a combination thereof. 
     
     
         42 . The method of  claim 27 , wherein the ellagitannin composition, the enzymatic composition, or both is formulated for oral administration. 
     
     
         43 . The method of  claim 27 , wherein the enzymatic composition, ellagitannin composition, or both are administered as a food, a beverage, a feed composition, or a nutritional supplement. 
     
     
         44 . The method of  claim 27 , wherein the enzymatic composition, ellagitannin composition, or both are administered as a liquid, syrup, tablet, troche, gummy, capsule, powder, gel, or film. 
     
     
         45 . The method of  claim 27 , wherein the enzymatic composition, ellagitannin composition, or both comprise a pharmaceutically acceptable carrier. 
     
     
         46 . The method of  claim 45 , wherein the enzymatic composition, ellagitannin composition, or both are in an enteric-coated formulation. 
     
     
         47 . The method of  claim 27 , wherein the enzymatic composition includes at least 10 5  CFU of the EES microbe. 
     
     
         48 . The method of  claim 42 , wherein the formulation for oral administration is a diary product. 
     
     
         49 . The method of  claim 27 , wherein the EES microbe or extract thereof comprises one or more ellagitannin enzymes which comprise a tannin acyl hydrolase enzyme, a gallate decarboxylase enzyme, or a combination thereof.