Methods of screening for microorganisms that impart beneficial properties to plants

Abstract

The present invention relates to methods for the screening, identification and/or application of microorganisms and/or compositions of use in imparting beneficial properties to plants, and microorganisms and compositions identified therefrom.

61 . A method for the selection of one or more microorganisms capable of improving resistance or tolerance of one or more plants to an abiotic stressor, the method comprising the steps of:
 a) subjecting one or more plants to a growth medium in the presence of a first set of one or more microorganisms, wherein the growth medium comprises the abiotic stressor;   b) selecting one or more plant parts from the one or more plants of step a), wherein the selection is based on increased resistance or tolerance of the one or more plants to the abiotic stressor;   c) acquiring a second set of one or more microorganisms from said one or more plant parts selected in step b); and   d) repeating steps a) to c) one or more times, wherein the second set of one or more microorganisms acquired in step c) is used as the first set of microorganisms in step a) of any successive repeat.   
     
     
         62 . The method according to  claim 61 , wherein the step of subjecting one or more plants to a growth medium involves growing or multiplying the plant. 
     
     
         63 . The method according to  claim 61 , wherein one or more plants are subjected to a growth medium in the presence of the first set of microorganisms. 
     
     
         64 . The method according to  claim 61 , wherein the abiotic stressor is drought or heat. 
     
     
         65 . The method according to  claim 61 , wherein the abiotic stressor is the presence of a metal or salt. 
     
     
         66 . The method according to  claim 61 , wherein a composition conferring abiotic stress is in the growth medium. 
     
     
         67 . The method according to claim  4 , wherein different selection criteria are used in different iterations of the method. 
     
     
         68 . The method according to  claim 61 , wherein the second set of one or more microorganisms are acquired in crude form. 
     
     
         69 . The method according to  claim 61 , wherein two or more microorganisms are acquired in step c), and the method further comprises separating the two or more microorganisms into individual isolates, selecting two or more individual isolates, and then combining the selected two or more individual isolates. 
     
     
         70 . The method according  claim 69 , wherein the combined isolates are used as the first set of one or more microorganisms in step a) of any successive repeat of the method. 
     
     
         71 . The method according to  claim 61 , wherein two or more methods of the invention may be performed separately and the second set of one or more microorganisms acquired in step c) of each separate method are combined. 
     
     
         72 . The method according to  claim 71 , wherein the combined microorganisms are used as the first set of one or more microorganisms in step a) of any successive repeat of the method. 
     
     
         73 . The method according to  claim 61 , wherein plant material is used as the source of microorganisms for step a). 
     
     
         74 . The method according to  claim 61 , wherein the method selects for one or more unculturable microorganisms or one or more endophytes. 
     
     
         75 . A method for the production of a composition to support plant growth, quality, and/or health, the method comprising the method of  claim 61 , and the additional step of combining the one or more microorganisms selected by the method with one or more additional ingredients. 
     
     
         76 . A method for the production of a composition to suppress or inhibit the growth, quality, and/or health of a plant, the method comprising the method of  claim 61 , and the additional step of combining the one or more microorganisms selected by the method with one or more additional ingredients. 
     
     
         77 . A method for the selection of a composition which is capable of improving resistance or tolerance of one or more plants to a pest or pathogen, the method comprising at least the steps of:
 a) culturing one or more microorganisms selected by the method of  claim 61  in one or more medias to provide one or more cultures;   b) separating the one or more microorganisms from the one or more medias in the one or more cultures after a period of time to provide one or more compositions substantially free of microorganisms;   c) subjecting one or more plants to the one or more compositions of step b); and   d) selecting one or more compositions from step c) if it is observed to impart improved resistance or tolerance of the one or more plants of step c) to said pest or pathogen.   
     
     
         78 . A method for the selection of a composition which is capable of imparting resistance or tolerance of the one or more plants to a pest or pathogen, the method comprising at least the steps of:
 a) culturing one or more microorganisms selected by the method of  claim 61  in one or more medias to form one or more cultures;   b) inactivating the one or more cultures of step a) to provide one or more compositions containing one or more inactivated microorganisms;   c) subjecting one or more plants to the one or more compositions of step b); and   d) selecting one or more compositions from step c) if it is observed to impart improved resistance or tolerance of the one or more plants of step c) to said pest or pathogen.   
     
     
         79 . A method for the selection of one or more microorganisms which are capable of producing a composition which is capable of improving resistance or tolerance of one or more plants to a pest or pathogen, the method comprising at least the steps of:
 a) culturing one or more microorganisms selected by the method of  claim 61  in one or more medias to provide one or more cultures;   b) separating the one or more microorganisms from the one or more medias in the one or more cultures from step a) to provide one or more compositions substantially free of microorganisms;   c) subjecting one or more plants to the one or more composition from step b); and   d) selecting the one or more microorganisms associated with one or more compositions observed to impart improved resistance or tolerance to said pest or pathogen to the one or more plants.   
     
     
         80 . A method for the selection of one or more microorganisms which are capable of producing a composition which is capable of improving resistance or tolerance of one or more plants to a pest or pathogen, the method comprising at least the steps of:
 a) culturing one or more microorganisms selected by the method of  claim 61  in one or more medias to provide one or more cultures;   b) inactivating the one or more cultures of step a) to provide one or more compositions containing one or more inactivated microorganisms;   c) subjecting one or more plants to the one or more compositions of step b); and   d) selecting the one or more microorganisms associated with one or more compositions observed to impart improved resistance or tolerance to said pest or pathogen to the one or more plants.