Method and device for high throughput cell deformability measurements

Abstract

A system is disclosed that enables the automated measurement of cellular mechanical parameters at high throughputs. The microfluidic device uses intersecting flows to create an extensional flow region where the cells undergo controlled stretching. Cells are focused into streamlines prior to entering the extensional flow region. In the extensional region, each cell's deformation is measured with an imaging device. Automated image analysis extracts a range of independent biomechanical parameters from the images. These may include cell size, deformability, and circularity. The single cell data that is obtained may then be used to in a variety of ways. Scatter density plots of deformability and circularity may be developed and displayed for the user. Mechanical parameters such as deformability and circularity may be gated or thresholded to identify certain cells of interest or sub-populations of interest. Similarly, the mechanical data obtained using the device may be used as cell signatures.

1 . A system for measuring particle deformability comprising:
 a substrate containing first and second microfluidic channels dimensioned to carry cells therein;   an extensional region comprising an intersection of the first and second microfluidic channels, wherein the first and second microfluidic channels intersect in substantially opposite directions;   at least one outlet channel coupled to the extensional region;   an imaging device configured to capture a plurality of image frames of cells passing through the extensional region; and   at least one processor configured to calculate a morphological parameter of the particle passing through the extensional region.   
     
     
         2 - 31 . (canceled)