US2022032257A1PendingUtilityA1

Screening assays and methods

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Assignee: HARVARD COLLEGEPriority: Sep 16, 2005Filed: Sep 20, 2021Published: Feb 3, 2022
Est. expirySep 16, 2025(expired)· nominal 20-yr term from priority
Inventors:J. Christopher LoveHidde L. PloeghJehnna Ronan
G01N 33/6854B82Y 30/00B01J 2219/00675B01L 2200/025B01J 2219/00527G01N 33/577B01L 2200/12G01N 33/531B01L 3/50853B01J 2219/00734B01J 2219/0074G01N 21/6452B01J 2219/00725Y10T436/24G01N 33/5052B01L 2300/0819B01J 2219/00722Y10S436/809G01N 33/505G01N 33/543B01J 2219/00317B01J 2219/00585B01J 19/0046G01N 33/54366B01L 2300/12
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Claims

Abstract

Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.

Claims

exact text as granted — not AI-modified
What is claimed: 
     
         1 . A printed microarray of unknown cell-derived products, each position of said array comprising a deposit corresponding to a composition of a single cell, said deposit being less than 100 micrometers in diameter. 
     
     
         2 . The microarray of  claim 1 , wherein said deposit is a secreted cell-derived product selected from the group consisting of an antibody, cytokine, chemokine, and inflammatory mediator. 
     
     
         3 . The microarray of  claim 1 , wherein said microarray comprises a capture ligand for a class of secreted products. 
     
     
         4 . The microarray of  claim 3 , wherein said capture ligand binds to a single immunoglobulin isotype.

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