US2022033788A1PendingUtilityA1

Recombinant components and compositions for use in food products

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Assignee: PERFECT DAY INCPriority: Oct 17, 2018Filed: Oct 17, 2019Published: Feb 3, 2022
Est. expiryOct 17, 2038(~12.3 yrs left)· nominal 20-yr term from priority
Inventors:Timothy GeistlingerHeather JensenRavirajsinh JhalaHendrik MeermanBalakrishnan RameshTy WagonerTimothy Scott JohnsonVincent Wei-Xiang WuFrancesca Manea
C12R 2001/885C12P 21/02C12N 1/14C07K 14/4717C12N 9/16C12Y 301/01004C12P 21/06C12Y 301/01074A23J 1/205A23L 29/06C12N 9/18A23V 2002/00A23J 1/20A23J 1/009A23G 9/38C12Y 301/01005A23J 1/202C12N 9/20A23J 1/008C12Y 301/01006A23J 1/006C12Y 301/04003C12Y 301/01003A23L 27/00A23L 29/00C12Y 301/01001C12N 15/80A23J 3/20A23J 3/08
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Claims

Abstract

Provided are methods for producing food products comprising recombinant components, and compositions used in and food products produced by such methods.

Claims

exact text as granted — not AI-modified
What is claimed is: 
     
         1 - 104 . (canceled) 
     
     
         105 . A recombinant microbial host cell that is capable of producing a recombinant component and that comprises a genetic modification that decreases or essentially eliminates activity or expression of an esterase compared to activity or expression of the esterase comprised in a corresponding recombinant microbial host cell, wherein the esterase comprises a cutinase. 
     
     
         106 . The recombinant microbial host cell of  claim 105 , wherein the esterase consists of a cutinase. 
     
     
         107 . The recombinant microbial host cell of  claim 106 , wherein the cutinase comprises an amino acid sequence that is at least 80% identical to a sequence selected from UniProt sequences G0RH85, A2QBP1, A0A2T4BJB5, A5ABE6, A2R2W3, G0R6X2, and G2QH51. 
     
     
         108 . The recombinant microbial host cell of  claim 107 , wherein the cutinase comprises an amino acid sequence that is at least 80% identical to a sequence selected from UniProt sequence G0RH85. 
     
     
         109 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises a carboxylic ester hydrolase. 
     
     
         110 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises a triacylglycerol lipase. 
     
     
         111 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises a phospholipase A2. 
     
     
         112 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises a lysophospholipase. 
     
     
         113 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises an acetylesterase. 
     
     
         114 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises a phospholipase C. 
     
     
         115 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises a GDSL lipase. 
     
     
         116 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises an esterase that comprises an amino acid sequence that is at least 80% identical to a sequence selected from UniProt sequences G0RIU1, A2R1N7, G0RBM4, A0A2T4BBP9, G2Q379, A0A2T4AZ21, A2R8Z3, A2QPC2, G0RCG3, A0A2T4BCL7, A0A2T4B416, and A0A2T4BNI9. 
     
     
         117 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises an esterase that comprises an amino acid sequence that is at least 80% identical to a sequence selected from UniProt sequences G0R9X3 and G0RHJ4. 
     
     
         118 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises an esterase that comprises an amino acid sequence that is at least 80% identical to a sequence selected from UniProt sequences G0RX90, G2Q0K1, A0A2T4B235, A2QC75, G0RLB0, G0RLB7, G0RWS1, and A2QF42. 
     
     
         119 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises an esterase that comprises an amino acid sequence that is at least 80% identical to a sequence selected from UniProt sequences G0RJ76, G0RKL4, and G0RRK3. 
     
     
         120 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises an esterase that comprises an amino acid sequence that is at least 80% identical to a sequence selected from UniProt sequences G0REM9, A0A2T4BFY3, A2QAD7, G0RGQ7, G0RJC6, G0RQN5, G0RTR6, G0RBZ6, and G0RDK5. 
     
     
         121 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises an esterase that comprises an amino acid sequence that is at least 80% identical to a sequence selected from UniProt sequences G0R707, A2QM14, and A2R709. 
     
     
         122 . The recombinant microbial host cell of  claim 105 , wherein the esterase further comprises an esterase that comprises an amino acid sequence that is at least 80% identical to a sequence selected from UniProt sequences G0RBJ0, G0RJY0, and G0RMI3. 
     
     
         123 . The recombinant microbial host cell of  claim 105 , wherein the genetic modification is a genetic modification in a control sequence or a functional part thereof that drives expression of a transcription factor that regulates expression of the esterase or in a coding sequence that encodes such transcription factor or a functional part thereof. 
     
     
         124 . The recombinant microbial host cell of  claim 123 , wherein the transcription factor comprises an amino acid sequence that is at least 80% identical to a sequence selected from UniProt sequences G0RX49, G0RHG1, A0A2T4B416, A0A2T4BJU6, A2R2J1, A2R903, G2Q2Z5, and G2Q8I6. 
     
     
         125 . The recombinant microbial host cell of  claim 105 , wherein the recombinant microbial host cell is selected from the group consisting of a recombinant bacterial host cell, a recombinant yeast host cell, and a recombinant filamentous fungal host cell. 
     
     
         126 . The recombinant microbial host cell of  claim 125 , wherein the recombinant filamentous fungal host cell is derived from a filamentous fungal host cell selected from the group consisting of an  Aspergillus niger  host cell, a  Myceliophthora thermophila  host cell, a  Trichoderma reesei  host cell, and a  Trichoderma citrinoviride  host cell. 
     
     
         127 . The recombinant microbial host cell of  claim 105 , wherein the recombinant component is a recombinant protein. 
     
     
         128 . The recombinant microbial host cell of  claim 127 , wherein the recombinant protein is selected from the group consisting of a recombinant plant protein, a recombinant algae protein, a recombinant fungal protein, a recombinant microbial protein, and a recombinant animal protein. 
     
     
         129 . The recombinant microbial host cell of  claim 128 , wherein the recombinant animal protein is a recombinant milk protein. 
     
     
         130 . The recombinant microbial host cell of  claim 129 , wherein the recombinant milk protein is a recombinant whey protein. 
     
     
         131 . The recombinant microbial host cell of  claim 130 , wherein the recombinant whey protein is a recombinant β-lactoglobulin. 
     
     
         132 . The recombinant microbial host cell of  claim 130 , wherein the recombinant whey protein is a recombinant α-lactalbumin. 
     
     
         133 . The recombinant microbial host cell of  claim 129 , wherein the recombinant milk protein is a recombinant casein. 
     
     
         134 . The recombinant microbial host cell of  claim 133 , wherein the recombinant casein is a recombinant κ-casein. 
     
     
         135 . The recombinant microbial host cell of  claim 133 , wherein the recombinant casein is a recombinant β-casein.

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