Brassica carinata varieties producing seed with reduced glucosinolate content

Abstract

The invention is in the field of Brassica carinata breeding (i.e. Ethiopian mustard breeding), specifically to Brassica carinata varieties producing seed having reduced total glucosinolate content relative to seed from existing Brassica carinata commercial varieties when grown in the same location under the same conditions. The present invention relates to seeds, plants or parts thereof, cells, methods of making, and uses of such Brassica carinata varieties and their progeny.

1 . A  Brassica carinata  variety, wherein the variety produces seed having a total glucosinolate content that is reduced by at least 14% relative to the total glucosinolate content of seed produced by a commercial or check variety of  Brassica carinata  grown in the same location under the same environmental conditions. 
     
     
         2 . A  Brassica carinata  variety, wherein the variety produces seed having a total glucosinolate content that is reduced by at least 8 μmol per g of seed relative to the total glucosinolate content of seed produced by a commercial or check variety of  Brassica carinata  grown in the same location under the same environmental conditions. 
     
     
         3 . The  Brassica carinata  variety of  claim 1 , wherein the  Brassica carinata  variety is
 (a) DH-146.047, representative seed of which having been deposited under NCIMB accession number 43005;   (b) DH-146.194, representative seed of which having been deposited under NCIMB accession number 43006;   (c) DH-146.214, representative seed of which having been deposited under NCIMB accession number 43008; or   (d) DH-146.842, representative seed of which having been deposited under NCIMB accession number 43009.   
     
     
         4 . The  Brassica carinata  variety of  claim 3 , wherein the  Brassica carinata  variety is DH-146.842, representative seed of which having been deposited under NCIMB accession number 43005. 
     
     
         5 . A seed, plant, plant part, or cell of the  Brassica carinata  variety of  claim 1 . 
     
     
         6 . A tissue culture of protoplasts or regenerable cells of the plant of  claim 5 . 
     
     
         7 . A method of producing a  Brassica carinata  variety having a desired trait, wherein the method comprises
 (a) crossing a plant of the  Brassica carinata  variety of  claim 1  with a plant of a different  Brassica carinata  variety having a desired trait to produce F1 hybrid seed; and   (b) growing the resultant F1 hybrid seed and selecting one or more progeny plants having the desired trait and at least a portion of the genetic make up of the  Brassica carinata  variety of  claim 1 ;   (c) backcrossing the selected progeny plants with a plant of the  Brassica carinata  variety of  claim 1 , or with the different  Brassica carinata  plant having a desired trait, to produce backcross progeny seed;   (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait and at least a portion of the genetic make up of the  Brassica carinata  variety of  claim 1 .   
     
     
         8 . The method of  claim 7 , further comprising repeating steps (c) and (d) until the  Brassica carinata  variety has the desired trait and produces seed having a total glucosinolate content that is reduced by at least 14% relative to the total glucosinolate content of seed produced by a commercial or check variety of  Brassica carinata  grown in the same location under the same environmental conditions. 
     
     
         9 . A method of producing a  Brassica carinata  variety having a desired trait, wherein the method comprises
 (a) crossing a plant of the  Brassica carinata  variety of  claim 2  with a plant of a different  Brassica carinata  variety having a desired trait to produce F1 hybrid seed; and   (b) growing the resultant F1 hybrid seed and selecting one or more progeny plants having the desired trait and at least a portion of the genetic make up of the  Brassica carinata  variety of  claim 2 ;   (c) backcrossing the selected progeny plants with a plant of the  Brassica carinata  variety of  claim 2 , or with the different  Brassica carinata  plant having a desired trait, to produce backcross progeny seed;   (d) growing the resultant backcross progeny seed and selecting backcross progeny plants that have the desired trait and at least a portion of the genetic make up of the  Brassica carinata  variety of  claim 2 .   
     
     
         10 . The method of  claim 9 , further comprising repeating steps (c) and (d) until the  Brassica carinata  variety has the desired trait and produces seed having a total glucosinolate content that is reduced by at least 8 μmol per g of seed relative to the total glucosinolate content of seed produced by a commercial or check variety of  Brassica carinata  grown in the same location under the same environmental conditions. 
     
     
         11 . A plant, or part thereof, or seed thereof, produced by the method of  claim 7 . 
     
     
         12 . A method for producing a first generation (F1) hybrid  Brassica carinata  seed comprising crossing a plant of the  Brassica carinata  variety of  claim 1  with a different  Brassica carinata  plant and harvesting the resultant F1 hybrid  Brassica carinata  seed, and wherein the plant of the  Brassica carinata  variety of  claim 1  is either a female parent or a male parent. 
     
     
         13 . A method for producing a first generation (F1) hybrid  Brassica carinata  seed comprising crossing a plant of the  Brassica carinata  variety of  claim 2  with a different  Brassica carinata  plant and harvesting the resultant F1 hybrid  Brassica carinata  seed, and wherein the plant of the  Brassica carinata  variety of  claim 2  is either a female parent or a male parent. 
     
     
         14 . An F1 hybrid seed produced by the method of  claim 12 . 
     
     
         15 . An F1 hybrid plant produced from the F1 hybrid seed of  claim 14 . 
     
     
         16 . A method for producing a Double Haploid variety comprising:
 (a) isolating a flower bud of the F1 plant of  claim 15 ;   (b) dissecting out a haploid microspore;   (c) placing the haploid microspore in culture;   (d) inducing the microspore to differentiate into an embryo and subsequently into a plantlet;   (e) identifying whether the plantlet contains a diploid chromosome number, wherein the diploid chromosome number occurred through chromosome doubling; and   (f) continuing to grow the plantlet if it contains a diploid chromosome number.   
     
     
         17 . A commercial plant product produced from a plant of the  Brassica carinata  variety of  claim 1 . 
     
     
         18 . The commercial plant product of  claim 17 , wherein the commercial plant product comprises oil, meal, protein isolate, or biofumigant. 
     
     
         19 . The commercial plant product of  claim 18 , wherein the commercial plant product comprises meal or protein isolate having a total glucosinolate content of no more than 30 micromoles per gram (dry weight). 
     
     
         20 . Crushed, non-viable seed produced by the  Brassica carinata  variety of  claim 1 .