Systems and methods for run-time sequencing run quality monitoring

Abstract

Systems and methods of providing run-time quality control and monitoring of a single or multiple sequencing runs are provided herein. In some embodiments, the run-time system includes or is in communication with a processor capable of determining various types of run-time information relating to the quality, progress, etc. of various sequencing runs. In some embodiments, the system can also be in communication with a user interface, for example, a GUI, capable of representing and communicating various types of information to a user regarding the quality of the individual or multiple runs, the functioning of the instrument, an error event, etc. Additionally, the system can capable of receiving actionable information from a user via the GUI thereby allowing the user to terminate or repeat various sequencing steps in a particular run, terminate a entire run, terminate all runs, allow a run to proceed, etc.

What is claimed: 
     
         1 . A computer program product, comprising a non-transitory computer-readable storage medium whose contents include a program with instructions being executed on a processor, the instructions comprising:
 instructions to determine a duration for sequencing a nucleic acid sample by a sequencing instrument;   instructions to display a graph indicating the duration;   instructions to monitor the process of the sequencing and display the progress on the graph;   instructions to determine a data quality for the sequencing;   instructions to indicate an event condition on the graph if the data quality falls below a predefined threshold.   
     
     
         2 . The computer program product of  claim 1 , further comprising:
 instructions to determine a required amount of a reagent needed by a sequencing instrument to sequence a nucleic acid sample;   instructions to determine an available amount of the reagent;   instructions to identify a time when the sequencing instrument will need an additional amount of the reagent if the required amount exceeds the available amount;   instructions to schedule an event prior to the time, the event including instructing the sequencing instrument to stop sequencing the nucleic acid, notifying an operator to add an additional amount of the reagent, and instructing the sequencing instrument to continue sequencing after the additional amount is added.   
     
     
         3 . The computer program product of  claim 2 , further comprising instructions to indicate the event on the graph. 
     
     
         4 . The computer program product of  claim 2 , further comprising instructions to display a representation of a reagent storage area of the sequencing instrument to indicate where the additional amount of the reagent should be added. 
     
     
         5 . The computer program product of  claim 2 , wherein the graph is a timeline.