Agent for promoting expression of n-acetylgalactosaminyltransferase containing extract from inflamed tissues inoculated with vaccinia virus

Abstract

An object of the present invention is to provide an agent for promoting expression of N-acetylgalactosaminyltransferase that contains an extract from inflamed tissues inoculated with vaccinia virus. The present invention demonstrated that the extract from inflamed tissues inoculated with vaccinia virus promotes the expression of N-acetylgalactosaminyltransferase in intervertebral disc cells. Thus, the extract from inflamed tissues inoculated with vaccinia virus or a preparation containing the extract is useful as an agent for promoting the expression of N-acetylgalactosaminyltransferase in intervertebral disc cells.

1 . A method for determining or evaluating an extract from inflamed tissues inoculated with vaccinia virus or a preparation containing the extract, wherein the action of promoting expression of an N-acetylgalactosaminyltransferase in intervertebral disc cells is used as an index. 
     
     
         2 . The method according to  claim 1 , wherein the N-acetylgalactosaminyltransferase is N-acetylgalactosaminyltransferase 1. 
     
     
         3 . The method according to  claim 1 , wherein the N-acetylgalactosaminyltransferase is N-acetylgalactosaminyltransferase 2. 
     
     
         4 . The method according to  claim 1 , comprising
 comparing expression increase rates between the N-acetylgalactosaminyltransferase 1 and the N-acetylgalactosaminyltransferase 2, and   confirming that the N-acetylgalactosaminyltransferase 1 has a higher expression increase rate.   
     
     
         5 . The method according to  claim 1 , wherein the inflamed tissues are inflamed skin tissues of rabbits. 
     
     
         6 . A method for verifying that the extract from inflamed tissues inoculated with vaccinia virus or the preparation satisfies the quality standard by performing the determination or evaluation of  claim 1 . 
     
     
         7 . The method according to  claim 6 , wherein the preparation is an injectable preparation or an oral preparation.