Stabilized thrombin

Abstract

The present invention is directed to compounds, methods for stabilizing thrombin activity with a thrombin binding oligonucleotide and to stabilized thrombin. The thrombin binding oligonucleotide is capable of inhibiting thrombin activity whereby the inhibition can be reversed with an antisense oligonucleotide.

1 - 33 . (canceled) 
     
     
         34 . A delivery applicator comprising:
 a barrel holding an oligonucleotide inhibited thrombin; and   a vessel having a delivery opening and holding an antisense oligonucleotide;   wherein the barrel and the vessel are capable of being in fluid communication, so that after fluid communication and contact of the inhibited thrombin with the antisense, thrombin activity is increased before delivering thrombin through the delivery opening.   
     
     
         35 . The delivery applicator of  claim 34 , wherein the fluid communication is via a re-sealable opening positioned between the barrel and the vessel. 
     
     
         36 . The delivery applicator of  claim 34 , further comprising a barrel having a delivery opening and holding fibrinogen. 
     
     
         37 . The delivery applicator of  claim 34 , wherein the antisense is bound to a solid phase. 
     
     
         38 . A delivery applicator comprising:
 a container holding an oligonucleotide inhibited thrombin; and an antisense oligonucleotide, wherein the oligonucleotide inhibited thrombin and the antisense oligonucleotide are held in separate chambers that are capable of being in fluid communication, so that allowing fluid communication between the chambers and contact between the inhibited thrombin and the antisense results in increased thrombin activity before delivery, and wherein the container has an opening for delivery therethrough of the thrombin.   
     
     
         39 . The delivery applicator of  claim 38 , wherein the antisense is bound to a solid phase. 
     
     
         40 . (canceled)