Pdgf receptor beta binding polypeptides

Abstract

The present invention provides binding polypeptides (e.g., antibodies or fragments thereof) that specifically bind to a target antigen (e.g., a human antigen, e.g., human PDGFRβ) with high affinity. The invention also provides, libraries of binding polypeptides, pharmaceutical compositions, as well as nucleic acids encoding binding polypeptides, recombinant expression vectors and host cells for making such binding polypeptides. Methods of using binding polypeptide of the invention to diagnose and treat disease are also encompassed by the invention.

1 .- 39 . (canceled) 
     
     
         40 . A diverse library of unpaired VH domains, wherein each member of the library binds to human PDGFRβ, and wherein the library is a nucleic acid display library. 
     
     
         41 . The library of  claim 40 , wherein the nucleic acid display library is a DNA display library. 
     
     
         42 . The library of  claim 40 , wherein diversity lies in the FR1-FR3 regions, and wherein each member of the library comprises the CDR3 amino acid sequence set forth in SEQ ID NO: 1. 
     
     
         43 . A diverse library of stable VH/VL pairs, wherein each member of the library binds to human PDGFRβ, and wherein the library is a nucleic acid display library. 
     
     
         44 . The library of  claim 43 , wherein the nucleic acid display library is a DNA display library. 
     
     
         45 . The library of  claim 43 , wherein each member of the library comprises a VH domain comprising the CDR3 amino acid sequence set forth in SEQ ID NO: 1. 
     
     
         46 . The library of  claim 45 , wherein the VL domains are human VL domains.