Bone-like prosthetic implants

Abstract

A prosthetic implant comprising a biocompatible three-dimensional scaffold and at least two cell types selected from the group consisting of osteoblasts, osteoclasts, and endothelial cells or progenitors thereof.

1 . A composition comprising an injectable medium and a mineral implant having a biocompatible, three-dimensional mineral scaffold comprising ex vivo expanded mesenchymal cells and at least one cell type selected from the group consisting of: osteoblasts, osteoclasts, chondrocytes, endothelial cells and progenitors thereof, wherein said mesenchymal cells and said at least one cell type are organized in more than one layer in a 3-D interaction, wherein said mesenchymal cells comprise a combination of osteoprogenitor cells and mesenchymal stem cells. 
     
     
         2 . The composition of  claim 1 , further comprising osteoblasts, osteoclasts, chondrocytes, endothelial cells, or any combination thereof. 
     
     
         3 . The composition of  claim 1 , wherein said mineral implant comprises an extracellular matrix. 
     
     
         4 . The composition of  claim 1 , wherein said mesenchymal cells and at least one cell type are at a density of at least 10 6  cells/ml in said implant. 
     
     
         5 . The composition of  claim 1 , wherein said scaffold comprises a bone particle selected from the group consisting of: a dry bone particle, a frozen bone particle and a dematerialized bone particle. 
     
     
         6 . The composition of  claim 1 , wherein said scaffold comprises a material selected from the group consisting of: calcium phosphate derivatives, calcium sulfate derivatives, calcium hydroxyapatite, silicate matrices, hydroxyapatite, and beta-3 calcium phosphate. 
     
     
         7 . The composition of  claim 1 , wherein said scaffold comprises a coating layer. 
     
     
         8 . The composition of  claim 1 , comprising a material selected from the group consisting of: poly-D-lysine, collagen, fibronectin, hydrogel, or a combination thereof. 
     
     
         9 . The composition of  claim 1 , wherein said scaffold has a pore size in the range of from about 50 microns to about 2000 microns. 
     
     
         10 . A method for repairing a bone lesion in a subject in need thereof, comprising inserting the composition of  claim 1  into said bone lesion, thereby repairing a bone lesion in a subject in need thereof. 
     
     
         11 . The method of  claim 10 , wherein said cells further comprise osteoblasts, osteoclasts, chondrocytes, endothelial cells, or any combination thereof. 
     
     
         12 . The method of  claim 10 , wherein said cells are at a density of at least 10 6  cells/ml of said implant. 
     
     
         13 . The method of  claim 10 , wherein said cells are arranged within more than one layer. 
     
     
         14 . The method of  claim 10 , wherein said cells are derived from bone marrow, placenta, adipose tissue, cord blood, cord vein, peripheral blood, mobilized peripheral blood, embryonic stem cells, or any combination thereof. 
     
     
         15 . The method of  claim 10 , wherein said scaffold has a pore size in the range of from about 50 microns to about 2000 microns. 
     
     
         16 . The method of  claim 10 , wherein said scaffold comprises a bone particle selected from the group consisting of: a dry bone particle, a frozen bone particle a dematerialized bone particle, or any combination thereof. 
     
     
         17 . The method of  claim 10 , wherein said scaffold comprises: a calcium phosphate derivative, a calcium sulfate derivative, calcium hydroxyapatite, a silicate matrice, hydroxyapatite, beta-3 calcium phosphate, or any combination thereof. 
     
     
         18 . The method of  claim 10 , wherein said composition further comprises poly-D-lysine, collagen, fibronectin, extra-cellular-matrix, hydrogel, or any combination thereof.