US2022112454A1PendingUtilityA1

Biologicals and their use in plants

54
Assignee: DANISCO US INCPriority: May 16, 2016Filed: Dec 22, 2021Published: Apr 14, 2022
Est. expiryMay 16, 2036(~9.8 yrs left)· nominal 20-yr term from priority
Inventors:Anne-Sophie Madeleine Elisabeth AldiguierJacob Andrew LatoneNicolas LeivaJoshua Klaus MichelAleksandra Virag
C12N 1/145A01N 63/30C12R 2001/645C12N 1/14
54
PatentIndex Score
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Claims

Abstract

Entomopathogenic fungal strains, compositions, and methods and compositions of producing and using the strains for reducing overall insect damage.

Claims

exact text as granted — not AI-modified
1 .- 28 . (canceled) 
     
     
         29 . A method of producing a fungal entomopathogenic product comprising:
 a. obtaining aerial conidospores of a fungal entomopathogen;   b. inoculating the aerial conidiospores into a liquid medium to generate a fungal entomopahogenic seed culture by fermentation; and   c. inoculating the fungal entomopathogen seed culture into a liquid medium to generate a fungal entomopahogenic product by fermentation, wherein the liquid medium comprises a first carbon source, a second carbon source, and a nitrogen source.   
     
     
         30 . The method of  claim 29 , further comprising obtaining the fungal entomopathogenic product from the liquid medium. 
     
     
         31 . The method of  claim 29 , wherein the first carbon source is in a limiting concentration to facilitate a non-optimal or stress condition of the fungal entomopathogen. 
     
     
         32 . The method of  claim 31 , wherein the first carbon source comprises 10 to 35 percent by weight/volume of the total medium. 
     
     
         33 . The method of  claim 29 , wherein the second carbon source is in a non-limiting concentration. 
     
     
         34 . The method of  claim 29 , wherein the second carbon source creates a non-optimal or stress condition of the fungal entomopathogen. 
     
     
         35 . The method of  claim 29 , wherein the first carbon source comprises a glucose molecule. 
     
     
         36 . The method of  claim 29 , wherein the second carbon source comprises a fructose, a galactose, a sorbitol, a sorbose, a sucrose, an arabinose, a maltodextrin, a ribose, or a xylose molecule. 
     
     
         37 . The method of  claim 29 , further comprising adjusting a fermentation parameter to create a non-optimal or stress condition that results in a change of a physiological state of the fungal entomopathogen. 
     
     
         38 . The method of  claim 30 , wherein obtaining the fungal entomopathogenic product from the liquid medium comprises vacuum drying. 
     
     
         39 . The method of  claim 29 , wherein obtaining aerial conidospores of a fungal entomopathogen comprises first generating aerial conidiospores of the fungal entomopathogen on agar media or solid state media. 
     
     
         40 . The method of  claim 29 , wherein the fungal entomopathogenic product comprises a spore, a conidiospore, a blastospore, an aerial conidiospore, a submerged conidiospore, a submerged blastospore, and/or a microsclerotium. 
     
     
         41 . The method of  claim 29 , wherein the fungal entomopathogen is selected from the group consisting of:  Metarhizium anisopliae  15013-1 , Metarhizium robertsii  23013-3, and  Metarhizium anisopliae  3213-1. 
     
     
         42 .- 53 . (canceled) 
     
     
         54 . A method of producing a fungal entomopathogenic product comprising inoculating a fungal entomopathogen strain into a liquid medium to generate a fungal entomopahogenic product by fermentation, wherein the liquid medium comprises a carbon source and a nitrogen source. 
     
     
         55 . The method of  claim 54 , further comprising obtaining the fungal entomopathogenic product from the liquid medium. 
     
     
         56 . The method of  claim 54 , further comprising a second carbon source. 
     
     
         57 . The method of  claim 56 , wherein the first carbon source is in a limiting concentration to facilitate a non-optimal or stress condition of the fungal entomopathogen. 
     
     
         58 . The method of  claim 56 , wherein the first carbon source comprises 10 to 35 percent by weight/volume of the total medium. 
     
     
         59 . The method of  claim 56 , wherein the second carbon source is in a non-limiting concentration. 
     
     
         60 . The method of  claim 56 , wherein the second carbon source creates a non-optimal or stress condition of the fungal entomopathogen. 
     
     
         61 . The method of  claim 56 , wherein the first carbon source comprises a glucose molecule. 
     
     
         62 . The method of  claim 56 , wherein the second carbon source comprises a fructose, a galactose, a sorbitol, a sorbose, a sucrose, an arabinose, a maltodextrin, a ribose, or a xylose molecule. 
     
     
         63 . The method of  claim 54 , further comprising adjusting a fermentation parameter to create a non-optimal or stress condition that results in a change of a physiological state of the fungal entomopathogen. 
     
     
         64 . The method of  claim 55 , wherein obtaining the fungal entomopathogenic product from the liquid medium comprises vacuum drying. 
     
     
         65 . The method of  claim 54 ,  55 , or  611 , wherein the fungal entomopathogenic product comprises a spore, a conidiospore, a blastospore, an aerial conidiospore, a submerged conidiospore, a submerged blastospore, and/or a microsclerotium. 
     
     
         66 . The method of  claim 55 , wherein the fungal entomopathogenic product is selected from the group consisting of:  Metarhizium anisopliae  15013-1 , Metarhizium robertsii  23013-3, and  Metarhizium anisopliae  3213-1. 
     
     
         67 . A method of producing a fungal entomopathogen comprising:
 a. inoculating a fungal entomopahogen into a liquid medium to generate a fungal entomopahogenic product by fermentation, wherein the liquid medium comprises a carbon source and a nitrogen source; and   b. adjusting a fermentation parameter to create a non-optimal or stress condition that results in a change of a physiological state of the fungal entomopathogen.   
     
     
         68 . The method of  claim 67 , wherein the fermentation parameter is selected from of the group consisting of: pH level, carbon dioxide evolution rate, dissolved oxygen percentage, agitation profile, and carbon source feed rate. 
     
     
         69 . The method of  claim 67 , further comprising obtaining the fungal entomopathogenic product from the liquid medium. 
     
     
         70 . The method of  claim 67 , wherein the carbon source is in a limiting concentration to facilitate a non-optimal or stress condition of the fungal entomopathogen. 
     
     
         71 . The method of  claim 70 , wherein the carbon source comprises 10 to 35 percent by weight/volume of the total medium. 
     
     
         72 . The method of  claim 67 , wherein the carbon source comprises a second carbon source and wherein the second carbon source is in a non-limiting concentration. 
     
     
         73 . The method of  claim 72 , wherein the second carbon source creates a non-optimal or stress condition of the fungal entomopathogen. 
     
     
         74 . The method of  claim 67 , wherein the carbon source comprises a glucose molecule. 
     
     
         75 . The method of  claim 67 , wherein the carbon source comprises a second carbon source and wherein the second carbon source comprises a fructose, a galactose, a sorbitol, a sorbose, a sucrose, an arabinose, a maltodextrin, a ribose, or a xylose molecule. 
     
     
         76 . The method of  claim 69 , wherein obtaining the fungal entomopathogenic product from the liquid medium comprises vacuum drying. 
     
     
         77 . The method of  claim 67 , wherein the fungal entomopathogenic product comprises a spore, a conidiospore, a blastospore, an aerial conidiospore, a submerged conidiospore, a submerged blastospore, and/or a microsclerotium. 
     
     
         78 . (canceled) 
     
     
         79 . A composition comprising a fungal entomopathogen and an agricultural acceptable carrier, wherein the fungal entomopathogen is produced by the method of  claim 29 . 
     
     
         80 . A composition comprising a fungal entomopathogen and an agricultural acceptable carrier, wherein the fungal entomopathogen is produced by the method of  claim 54 . 
     
     
         81 . A composition comprising a fungal entomopathogen and an agricultural acceptable carrier, wherein the fungal entomopathogen is produced by the method of  claim 67 .

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