US2022119783A1PendingUtilityA1

Recombinant host cells for the production of malonate

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Assignee: LYGOS INCPriority: Mar 6, 2012Filed: Dec 29, 2021Published: Apr 21, 2022
Est. expiryMar 6, 2032(~5.6 yrs left)· nominal 20-yr term from priority
Inventors:Jeffrey A. DietrichJeffrey L. FortmanEric J. Steen
C12N 9/16C07C 67/31C12P 7/46C07C 67/08C12Y 301/02004C07C 51/38C12N 1/20C12N 1/16
76
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Claims

Abstract

Systems and methods for the production of malonate in recombinant host cells.

Claims

exact text as granted — not AI-modified
1 .- 15 . (canceled) 
     
     
         16 . A malonyl-CoA hydrolase having at least 75% identity with SEQ ID NO: 9. 
     
     
         17 . The malonyl-CoA hydrolase of  claim 16  that is a  Pseudomonas fulva  protein further comprising the mutation E95N. 
     
     
         18 . A recombinant  Pichia kudriavzevii  host cell that comprises one or both of:
 a heterologous  Pseudomonas fulva  malonyl-CoA hydrolase having at least 75% identity with SEQ ID NO: 9, and   a heterologous  Saccharomyces cerevisiae  (Sc) aldehyde dehydrogenase (ALD) selected from Sc ALD 2 to 6.   
     
     
         19 . The recombinant  Pichia kudriavzevii  host cell of  claim 18  that comprises a heterologous  Pseudomonas fulva  malonyl-CoA hydrolase having at least 75% identity with SEQ ID NO: 9. 
     
     
         20 . The recombinant  Pichia kudriavzevii  host cell of  claim 18  that comprises a heterologous  Saccharomyces cerevisiae  aldehyde dehydrogenase selected from ScALD 2 to 6. 
     
     
         21 . The recombinant  Pichia kudriavzevii  host cell of  claim 18  that comprises a heterologous  Pseudomonas fulva  malonyl-CoA hydrolase having at least 75% identity with SEQ ID NO: 9 and a heterologous  Saccharomyces cerevisiae  aldehyde dehydrogenase selected from ScALD 2 to 6. 
     
     
         22 . The recombinant  Pichia kudriavzevii  host cell of  claim 18  that comprises the heterologous  Saccharomyces cerevisiae  aldehyde dehydrogenase Sc ALD 6. 
     
     
         23 . The recombinant  Pichia kudriavzevii  host cell of  claim 20  that comprises the heterologous  Saccharomyces cerevisiae  aldehyde dehydrogenase Sc ALD 6. 
     
     
         24 . The recombinant  Pichia kudriavzevii  host cell of  claim 21  that comprises the heterologous  Saccharomyces cerevisiae  aldehyde dehydrogenase Sc ALD 6. 
     
     
         25 . The recombinant  Pichia kudriavzevii  host cell of  claim 18  that further comprises a heterologous acetyl-CoA carboxylase. 
     
     
         26 - 42 . (canceled)

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