Micro-fluidic chip, liquid loading method thereof and micro-fluidic system

Abstract

Provided is a micro-fluidic chip, including a first substrate and a second substrate opposite to each other. A liquid storage cavity is formed between the first substrate and the second substrate, and a liquid inlet hole penetrating through the first substrate in a thickness direction is formed in the first substrate. The first substrate includes a first electrode layer and a hydrophobic layer that are sequentially disposed in the thickness direction of the first substrate, and the first electrode layer is on a surface of the hydrophobic layer away from the second substrate. The second substrate includes an adjustment layer and a second electrode layer that are sequentially disposed in a thickness direction of the second substrate, and the second electrode layer is on a surface of the adjustment layer away from the first substrate. A micro-fluidic system and a control method of the micro-fluidic chip are also provided.

1 - 21 . (canceled) 
     
     
         22 . A micro-fluidic chip, comprising a first substrate and a second substrate opposite to each other, wherein a liquid storage cavity is between the first substrate and the second substrate, a liquid inlet hole penetrating through the first substrate in a thickness direction of the first substrate is in the first substrate, the first substrate comprises a first electrode layer and a hydrophobic layer that are sequentially disposed in the thickness direction of the first substrate, and the first electrode layer is on a surface of the hydrophobic layer away from the second substrate;
 wherein the second substrate comprises an adjustment layer and a second electrode layer that are sequentially disposed in a thickness direction of the second substrate, and the second electrode layer is on a surface of the adjustment layer away from the first substrate; and   wherein in a case where the adjustment layer is in an electric field having a predetermined strength, a surface of the adjustment layer facing the first substrate exhibits one of hydrophilicity and hydrophobicity, and in a case where the electric field having the predetermined strength is removed, the surface of the adjustment layer facing the first substrate exhibits another one of hydrophilicity and hydrophobicity.   
     
     
         23 . The micro-fluidic chip of  claim 22 , wherein a liquid storage recess is on a surface of the first substrate facing the second substrate, and the liquid inlet hole penetrates through a top wall of the liquid storage recess. 
     
     
         24 . The micro-fluidic chip of  claim 23 , wherein a shape of the top wall of the liquid storage recess is of a convex polygon, the top wall comprises at least one inner angle that is a non-right angle, and one of inner angles of the top wall that are non-right angles is a liquid inlet angle, the liquid inlet hole is at the liquid inlet angle, a plurality of side walls of the liquid storage recess are at respective sides of the top wall, and the plurality side walls are perpendicular to the top wall. 
     
     
         25 . The micro-fluidic chip of  claim 24 , wherein the top wall of the liquid storage recess is of a convex pentagon, the top wall comprises two inner angles that are right angles and adjacent to each other, and the liquid inlet angle is opposite to a side of the top wall between the two right angles. 
     
     
         26 . The micro-fluidic chip of  claim 22 , wherein the first substrate further comprises a convex plate on a surface of the first substrate away from the second substrate, and the liquid inlet hole penetrates through a portion of the first substrate where the convex plate is disposed. 
     
     
         27 . The micro-fluidic chip of  claim 22 , wherein the liquid inlet hole comprises a conical hole portion and a cylindrical hole portion that are coaxially arranged, the conical hole portion is at one end of the cylindrical hole portion away from the second substrate, and a hole diameter of the conical hole portion is gradually reduced in a direction from the first substrate to the second substrate. 
     
     
         28 . The micro-fluidic chip of  claim 22 , wherein the first substrate further comprises a first base substrate, and the first electrode layer is on the first base substrate. 
     
     
         29 . The micro-fluidic chip of  claim 22 , wherein a material of the hydrophobic layer is the same as a material of adjustment layer. 
     
     
         30 . The micro-fluidic chip of  claim 22 , wherein the second electrode layer comprises a plurality of second electrode bars, at least one of the plurality of second electrode bars is opposite to the liquid inlet hole, and any two adjacent second electrode bars of the plurality of second electrode bars are insulated and spaced apart from each other. 
     
     
         31 . The micro-fluidic chip of  claim 30 , wherein an insulating spacer layer is between the second electrode layer and the adjustment layer. 
     
     
         32 . The micro-fluidic chip of  claim 31 , wherein a thickness of the insulating spacer layer is larger than a thickness of the adjustment layer. 
     
     
         33 . The micro-fluidic chip of  claim 22 , wherein the adjustment layer is made of a fluorine-based material. 
     
     
         34 . The micro-fluidic chip of  claim 22 , wherein a thickness of the adjustment layer is between 50 nm and 800 nm. 
     
     
         35 . The micro-fluidic chip of  claim 22 , wherein the first substrate is connected to and sealed with the second substrate through a sealant. 
     
     
         36 . The micro-fluidic chip of  claim 22 , wherein in a first direction perpendicular to the thickness direction of the first substrate, a distance from the liquid inlet hole to one end of the liquid storage cavity is larger than a distance from the liquid inlet hole to another end of the liquid storage cavity. 
     
     
         37 . The micro-fluidic chip of  claim 22 , wherein a gas outlet hole penetrating through the first substrate in the thickness direction of the first substrate is in the first substrate. 
     
     
         38 . A micro-fluidic system, comprising a liquid loading device and the micro-fluidic chip of  claim 22 , wherein a liquid loading nozzle of the liquid loading device is capable of being inserted into the liquid inlet hole. 
     
     
         39 . A control method of a micro-fluidic chip, the micro-fluidic chip comprising a first substrate and a second substrate opposite to each other, wherein a liquid storage cavity is between the first substrate and the second substrate, a liquid inlet hole penetrating through the first substrate in a thickness direction of the first substrate is in the first substrate, the first substrate comprises a first electrode layer and a hydrophobic layer that are sequentially disposed in the thickness direction of the first substrate, and the first electrode layer is on a surface of the hydrophobic layer away from the second substrate; wherein the second substrate comprises an adjustment layer and a second electrode layer that are sequentially disposed in a thickness direction of the second substrate, and the second electrode layer is on a surface of the adjustment layer away from the first substrate; and wherein in a case where the adjustment layer is in an electric field having a predetermined strength, a surface of the adjustment layer facing the first substrate exhibits one of hydrophilicity and hydrophobicity, and in a case where the electric field having the predetermined strength is removed, the surface of the adjustment layer facing the first substrate exhibits another one of hydrophilicity and hydrophobicity,
 wherein the method comprises:   providing a first reference voltage to the first electrode layer, and providing a second reference voltage to a portion of the second electrode layer, so that a portion of the adjustment layer exhibits hydrophilicity, wherein the portion of the second electrode layer is opposite to the liquid inlet hole, and the portion of the adjustment layer is opposite to the liquid inlet hole; and   after liquid entering the liquid storage cavity through the liquid inlet hole is in contact with the portion of the adjustment layer opposite to the liquid inlet hole, providing the second reference voltage to a portion of the second electrode layer adjacent to the portion of the second electrode layer opposite to the liquid inlet hole, so that a portion of the adjustment layer adjacent to the portion of the adjustment layer opposite to the liquid inlet hole exhibits hydrophilicity.   
     
     
         40 . The control method of  claim 39 , further comprising: removing the second reference voltage applied to the portion of the second electrode layer opposite to the liquid inlet hole, after the liquid entering the liquid storage cavity through the liquid inlet hole is in contact with the portion of the adjustment layer opposite to the liquid inlet hole. 
     
     
         41 . An electronic apparatus, comprising:
 a storage device having an executable program stored thereon; and   one or more processors that, when executing the executable program, cause the one or more processors to implement the control method of  claim 39 .