Pancreatic endocrine cells, method for producing same, and transdifferentiation agent

Abstract

A method for producing pancreatic endocrine cells, the method including introducing one or more genes of a GLIS family or one or more gene products thereof and a Neurogenin3 gene or one or more gene products thereof into somatic cells.

1 - 12 . (canceled) 
     
     
         13 . Pancreatic endocrine cells produced by a method for producing pancreatic endocrine cells, the method including introducing one or more genes of a GUS family or one or more gene products thereof and a Neurogenin3 gene or one or more gene products thereof into somatic cells,
 wherein the pancreatic endocrine cells are produced without undergoing an iPS cell stage.   
     
     
         14 . The pancreatic endocrine cells according to  claim 13 , wherein the method includes further introducing a Pdx1 gene or one or more gene products thereof into the somatic cells. 
     
     
         15 . The pancreatic endocrine cells according to  claim 13 , wherein the one or more genes of the GLIS family or one or more gene products thereof are a GLIS1 gene or one or more gene products thereof. 
     
     
         16 . The pancreatic endocrine cells according to  claim 13 , wherein the somatic cells are selected from the group consisting of fibroblasts, mesenchymal stem cells, adipose tissue-derived interstitial cells, adipose tissue-derived interstitial stem cells, neural stem cells, hematopoietic stem cells, hepatic cells, epithelial cells, renal cells, macrophages, lymphocytes, and muscle cells. 
     
     
         17 . The pancreatic endocrine cells according to  claim 13 , wherein the pancreatic endocrine cells are β cells. 
     
     
         18 . The pancreatic endocrine cells according to  claim 13 , wherein the method introduces a GLIS1 gene or one or more gene products thereof and a Neurogenin3 gene or one or more gene products thereof into the somatic cells. 
     
     
         19 . The pancreatic endocrine cells according to  claim 14 , wherein the method introduces a GLIS1 gene or one or more gene products thereof, a Neurogenin3 gene or one or more gene products thereof, and a Pdx1 gene or one or more gene products thereof into the somatic cells. 
     
     
         20 . The pancreatic endocrine cells according to  claim 14 , wherein the method introduces a GLIS3 gene or one or more gene products thereof, a Neurogenin3 gene or one or more gene products thereof, and a Pdx1 gene or one or more gene products thereof into the somatic cells.