Enhanced Adaptor Ligation

Abstract

The present disclosure describes a method of adaptor ligation to the ends of the fragmented double-stranded DNA molecules.

What is claimed is: 
     
         1 . A composition comprising:
 a polymerase possessing nick translation activity, a ligase and a partially double stranded adapter comprising oligonucleotide 1 and oligonucleotide 2; wherein oligonucleotide 1 comprises a 5′ phosphate; and wherein the 5′ portion of oligonucleotide 1 is complementary to the 3′ portion of oligonucleotide 2, and wherein the 3′ portion of oligonucleotide 1 is not complementary to the 5′ portion of oligonucleotide 2.   
     
     
         2 . The composition of  claim 1 , wherein the polymerase is Taq polymerase or DNA Polymerase I and the ligase is  E. coli  DNA ligase or T4 DNA ligase. 
     
     
         3 . A composition comprising:
 a polymerase possessing nick translation activity, a ligase and a partially double stranded adapter comprising oligonucleotide 1 and oligonucleotide 2; wherein oligonucleotide 1 lacks a 5′ phosphate; and wherein the 5′ portion of oligonucleotide 1 is complementary to the 3′ portion of oligonucleotide 2, and wherein the 3′ portion of oligonucleotide 1 is not complementary to the 5′ portion of oligonucleotide 2.