Compositions and methods of use for treatment or improvement of the condition and appearance of skin

Abstract

The present invention relates to compositions, formulations and methods of use thereof for treating, preventing and improving the condition and aesthetic appearance of skin. The present invention also relates to compositions, formulations and methods of use thereof for treatment of dermis wounds, aged dermis, diseased dermis or damaged dermis. The present compositions and formulations comprise a peptide with an amino acid sequence of QHREDGS (SEQ ID NO: 1) and methods of use thereof.

1 . A method of treating or improving a condition or aesthetic appearance of skin on a subject in need thereof, the method comprising: topically administering to the skin a formulation comprising a peptide comprising an amino acid sequence of QHREDGS (SEQ ID NO: 1), in an amount effective to treat or improve the condition or aesthetic appearance of the skin. 
     
     
         2 . The method of  claim 1 , where the topical administration is for treating a wound on the subject, the formulation being topically administered to the wound and the amount of the formulation administered being effective to treat the wound, wherein the wound is a dermis wound, an aged dermis, or a diseased dermis or damaged dermis. 
     
     
         3 . The method of  claim 2 , wherein the method results in a healed wound substantially free of scars. 
     
     
         4 . The method of  claim 2 , wherein the dermis wound is selected from the group consisting of a surgical wound, a cosmetic wound, a burn wound, an accidental wound or a self-inflicted wound, and any combinations thereof. 
     
     
         5 . The method of  claim 4 , wherein the surgical wound is a deep incisional wound or a superficial incisional wound. 
     
     
         6 . The method of  claim 4 , wherein the cosmetic wound is ablative fractional or non-fractional laser wound or a microneedling wound. 
     
     
         7 . The method of  claim 4 , wherein the burn wound is a sunburn, a radiation burn, an open blister or a peeling wound. 
     
     
         8 . The method of  claim 1 , wherein the topical administration is for improvement of the condition or aesthetic appearance of skin, and the improvement of the condition or aesthetic appearance of the skin is at least one of: reducing dermatological signs of chronological aging, photo-aging, hormonal aging, or actinic aging; preventing or reducing the appearance of lines or wrinkles; reducing the noticeability of facial lines and wrinkles, facial wrinkles on the cheeks, forehead, perpendicular wrinkles between the eyes, horizontal wrinkles above the eyes, and around the mouth, marionette lines, and particularly deep wrinkles or creases; preventing, reducing, or diminishing the appearance or depth of lines or wrinkles; improving the appearance of suborbital lines or periorbital lines; reducing the appearance of crow's feet; rejuvenating or revitalizing skin, particularly aging skin; reducing skin fragility; preventing or reversing of loss of glycosaminoglycans or collagen; ameliorating the effects of estrogen imbalance; preventing skin atrophy; preventing, reducing, or treating hyperpigmentation; minimizing skin discoloration; improving skin tone, radiance, clarity or tautness; preventing, reducing, or ameliorating skin sagging; improving skin firmness, plumpness, suppleness or softness; improving procollagen or collagen production; improving skin texture or promoting retexturization; improving skin barrier repair or function; improving the appearance of skin contours; restoring skin luster and/or brightness; minimizing dermatological signs of fatigue or stress; resisting environmental stress; replenishing ingredients in the skin decreased by aging or menopause; improving communication among skin cells; increasing cell proliferation or multiplication; increasing skin cell metabolism decreased by aging or menopause; retarding cellular aging; improving skin moisturization; enhancing skin thickness; increasing skin elasticity or resiliency; enhancing exfoliation; improving microcirculation; or decreasing or preventing cellulite formation. 
     
     
         9 . The method of  claim 8 , wherein a dermatological sign of chronological aging is selected from a group consisting of rhytids, wrinkles, jowls, sun damage, dull appearance of skin, loss of skin tautness, keratosis, hyperpigmentation, melasma, solar lentigo, solar keratosis, dermatoheliosis, skin discoloration, and any combinations thereof. 
     
     
         10 . The method of  claim 1 , wherein the topical administration is for improvement of the condition or aesthetic appearance of the skin, and the improvement of the condition or aesthetic appearance of the skin is regenerating collagen in a skin area of the subject, the amount of formulation administered being effective to regenerate collagen in the skin area. 
     
     
         11 . The method of  claim 1 , wherein the topical administration is for improvement of the condition or aesthetic appearance of the skin, and the improvement of the condition or aesthetic appearance of the skin is preventing or decreasing wrinkles in the subject, the amount of the formulation administered being effective to prevent or decrease wrinkles. 
     
     
         12 . The method of  claim 1 , wherein the topical administration is for improvement of the condition or aesthetic appearance of the skin, and the improvement of the condition or aesthetic appearance of the skin is decreasing skin redness in the subject, the amount of formulation administered being effective to decrease skin redness. 
     
     
         13 . The method of  claim 12 , wherein the skin redness results from acne, contact irritation, skin sensitivity, rosacea, eczema, surgery, laser treatment, allergies, microdermabrasion, dermabrasion, or any combination thereof. 
     
     
         14 . The method of  claim 1 , wherein the formulation further comprises a topically acceptable vehicle, and wherein the topically acceptable vehicle is selected from hydroxyethyl cellulose, hydroxypropyl cellulose, methylcellulose, hydroxypropyl methylcellulose, sodium hyaluronate, hyaluronic acid, sodium carbomer, aloe gel, xanthan gum, cetyl alcohol, cetearyl alcohol, propanediol 1,3 glycerin, rosehip oil, shea butter, jojoba oil, castor oil, macadamia oil, argan oil, kukui nut oil, petrolatum, mineral oil, evening primrose oil, glyceryl stearate, Polysorbate 60, Cetearyl Olivate, Sorbitan Olivate, Sodium Stearyl Lactylate, Stearic Acid, PEG 100 Stearate, Benzyl Alcohol, Ethylhexylglycerin, Benzoic Acid, Sorbic Acid, Gluconolactone, Sodium Benzoate, Calcium Gluconate, and any combinations thereof. 
     
     
         15 . The method of  claim 1 , wherein the formulation further comprises a carrier, and wherein the carrier is selected from a group consisting of: hydrogel, glycerol, propylene glycol, polyethylene glycol, chitosan, alginate, agarose, polyethers, polyesters, methylcellulose, hyaluronan, collagen, laminin, matrigel, fibronectin, vitronectin, poly-1-lysine, proteoglycans, fibrin glue, gels made by decellularization of engineered and natural tissues, polyglycolic acid (PGA), polylactic acid (PLA) and combinations of PGA and PLA such as PLGA, poly c-caprolactone, polyvinyl alcohol (PVA), polyethylene glycol (PEG), methyl methacrylate, poly(methyl methacrylate) (PMMA), poly(-hydroxyethyl methacrylate) (PolyHEMA), poly(glycerol sebacate), self-assembling peptide hydrogels, AcN-RARADADARARADADA-CNH (SEQ ID NO.2), polyurethanes, poly(isopropylacrylamide), poly(N-isopropylacrylamide) [poly(NIPAM)], and any combinations thereof. 
     
     
         16 . The method of  claim 15 , wherein the peptide is conjugated to the carrier. 
     
     
         17 . The method of  claim 15 , wherein the formulation comprises 0.5-50 wt % carrier. 
     
     
         18 . The method of  claim 1 , wherein the formulation comprises 0.001-1 wt % peptide. 
     
     
         19 . Use of a formulation for treating or improving a condition or aesthetic appearance of skin on a subject in need thereof, the use comprising topically administering the formulation to the subject in an amount effective to treat or improve the condition or aesthetic appearance of the skin, the formulation comprising a peptide comprising an amino acid sequence of QHREDGS (SEQ ID NO: 1). 
     
     
         20 . A formulation for treating or improving a condition or aesthetic appearance of skin on a subject in need thereof by topically administering the formulation to the subject in an amount effective to treat or improve the condition or aesthetic appearance of the skin, the formulation comprising a peptide comprising an amino acid sequence of QHREDGS (SEQ ID NO: 1).