Conditioned medium and extracellular matrix compositions from cells cultured under hypoxic conditions

Abstract

The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.

What is claimed is: 
     
         1 . A method of reducing and/or preventing fibrous encapsulation of a medical device after implantation in a subject comprising applying a surface coating on the medical device,
 wherein the surface coating comprises a composition made by the method comprising culturing fibroblast cells under hypoxic conditions of about 1-5% oxygen on microcarrier beads or a three-dimensional surface in a suitable growth medium, for at least 2 weeks, thereby producing multipotent stem cells, wherein the multipotent stem cells produce and secrete into the growth medium the composition.   
     
     
         2 . The method of  claim 1 , wherein applying the surface coating promotes wound healing. 
     
     
         3 . The method of  claim 1 , wherein applying the surface coating increases the longevity and/or function of the medical device. 
     
     
         4 . The method of  claim 1 , wherein applying the surface coating reduces a number of foreign-body giant cells (FBGC) per fiber. 
     
     
         5 . The method of  claim 1 , wherein applying the surface coating reduces a number of inflammatory cells at an implantation site. 
     
     
         6 . The method of  claim 1 , wherein applying the surface coating reduces the thickness of a fibrous capsule. 
     
     
         7 . The method of  claim 1 , wherein applying the surface coating promotes healing without the formation of scar or adhesion at an implantation site.