Photocleavage method and apparatus to clean fluidic devices

Abstract

A method and system for improving throughput of a fluidic system such as a biopolymer analysis system by cleaning accumulated or clogging biopolymer from the fluidic system is disclosed. The method and system utilize a light energy source to photocleave the biopolymer molecules that may accumulate or aggregate in the fluidic system or clog a passageway. The accumulated biopolymer may be exposed to a light energy source for a sufficient period of time such that the biopolymer molecule is dosed with sufficient energy to photocleave the biopolymer molecules, thereby restoring the efficiency of and flow through the system.

1 . A method for enhancing fluid flow, the method comprising:
 moving biopolymer molecules into contact with at least one fluidic channel in or on a device, whereby clogging occurs in the device due to coiling or aggregation of the biopolymer molecules or adsorption to or tangling of the biopolymer molecules around the channels or any other nano- or micro-features inside the channel or fluidic device; and   directing a light source at a region of the device in which said clogging has occurred in a manner effective to photocleave biopolymer molecules that contribute to said clogging, thereby facilitating removal or reduction of said clogging.   
     
     
         2 . The method of  claim 1 , further comprising applying a motive force to fluid in the region of the device in a manner effective to flush the photocleaved biopolymer molecules from the region of the device comprising said clogging. 
     
     
         3 . The method of  claim 2 , wherein the motive force comprises an electrostatic force, a pneumatic force, a capillary force, or any combination thereof. 
     
     
         4 . The method of  claim 1 , wherein the biopolymer molecules comprise DNA or RNA. 
     
     
         5 . The method of  claim 1 , further comprising labeling the biopolymer molecules with an indicator to facilitate photocleavage of the biopolymer molecules when exposed to the light source, wherein the light source is configured to generate a light matched with the indicator to enhance photocleaving capabilities. 
     
     
         6 . The method of  claim 5 , wherein the indicator used to label the biopolymer molecules comprises one or more of YOYO-1, YOYO-3, TOTO, methylene blue, Cu, Rh, compounds useful for photodynamic therapy, and other photon absorbers capable of facilitating cleavage of the biopolymer upon irradiation with photons. 
     
     
         7 . The method of  claim 6 , wherein the indicator is YOYO-1 and the light source has a wavelength of one of about 473 nm or about 488 nm. 
     
     
         8 . The method of  claim 1 , further comprising detecting a clogged or reduced flow condition. 
     
     
         9 . The method of  claim 8 , wherein the detection comprises identifying that a transport of the biopolymer molecules through the device falls below a threshold transport value. 
     
     
         10 . The method of  claim 8 , wherein the detection comprises direct imaging of at least one fluidic channel in or on the device to indicate clogging. 
     
     
         11 . The method of  claim 1 , wherein a cleaning of the region of the device in which the clogging has occurred is implemented in an automatic fashion at one of a predetermined time or a predetermined transport threshold. 
     
     
         12 . The method of  claim 1 , further comprising positioning the light source in a manner effective to minimize exposure of the device other than the region of the device in which said clogging has occurred to the light source. 
     
     
         13 . An apparatus for enhancing fluid flow, the apparatus comprising:
 a light source configured to generate a light beam configured to photocleave biopolymer molecules; and   a controller configured to:
 control a movement of the biopolymer molecules into contact with at least one fluidic channel in or on a device, whereby clogging occurs in the device due to coiling or aggregation of the biopolymer molecules or adsorption to or tangling of the biopolymer molecules around the channels or other nano- or micro-patterned features inside the channel or fluidic device; 
 direct the light source at a region of the device in which a clog of biopolymer molecules has formed; and 
 activate the light source to generate the light beam for an amount of time to facilitate a photocleaving of the biopolymer molecules forming said clog. 
   
     
     
         14 . The apparatus of  claim 13 , further comprising a motive force generator configured to generate a motive force, wherein the controller is configured to control a movement of the biopolymer molecules via the generated motive force and wherein the controller is further configured to apply the motive force to fluid in the region of the device in which said clog occurred in a manner effective to flush the photocleaved biopolymer molecules from the region. 
     
     
         15 . The apparatus of  claim 14 , wherein the motive force comprises an electrostatic force, a pneumatic force, a capillary force, or any combination thereof. 
     
     
         16 . The apparatus of  claim 14 , wherein the motive force generator is configured to generate one of an electrostatic force or a pneumatic force. 
     
     
         17 . The apparatus of  claim 13 , wherein the light beam generated by the light source has a wavelength of one of about 473 nm or about 488 nm. 
     
     
         18 . The apparatus of  claim 13 , wherein the biopolymer molecules comprise DNA or RNA. 
     
     
         19 . The apparatus of  claim 13 , further comprising a detector configured to detect a clog or reduced flow condition through the at least one fluidic channel in or on the device. 
     
     
         20 . The apparatus of  claim 19 , wherein the detector is further configured to identify that a transport of biopolymer molecules through the at least one fluidic channel in or on the device falls below a threshold transport value. 
     
     
         21 . The apparatus of  claim 20 , wherein the detector is further configured to directly image the at least one fluidic channel in or on the device to indicate the clog. 
     
     
         22 . The apparatus of  claim 13 , wherein the controller is configured to operate the light source and enhance fluid flow in at automatic fashion at one of a predetermined time or a predetermined transport threshold. 
     
     
         23 . The apparatus of  claim 13 , further comprising an x-y-z translation motor configured to position the light source in a manner effective to minimize exposure of the device other than the region of the device in which a clog of biopolymer molecules has formed to the light source and further configured to allow positioning of the light source at any location in relation to the device. 
     
     
         24 . An apparatus for characterizing a biopolymer molecule, the apparatus comprising:
 a fluidic device comprising a detection region comprising at least one channel, and further comprising the biopolymer molecule;   a motive force generator to move biopolymer molecules into the detection region; wherein a clog of biopolymer molecules occurs in the fluidic device hampering further flow of new biopolymer molecules to the detection region;   a detection system for determining a characteristic of the biopolymer molecules in the detection region;   a light source set to deliver a light comprising a configuration for photocleaving the biopolymer molecules forming the clog;   a light delivery system to deliver the light to any region of the fluidic device;   a positioning system to target the detection system to the detection region for characterization of the biopolymer molecules, and to target the light comprising the photocleaving configuration to a region of the device where the clog has formed; and   a controller configured to:
 activate the motive force generator to move the biopolymer molecules into the detection region; 
 direct the detection system to the detection region; 
 activate the detection system to determine the characteristics of the biopolymer molecules; 
 direct the light source configured for photocleaving biopolymer molecules to the region where the clog has formed; 
 activate the light source to generate a light for photocleaving biopolymer molecules forming the clog; and 
 activate the motive force generator after activating the light source to flush out the photocleaved biopolymer molecules wherein additional new biopolymer molecules flow into the detection region for characterization after the motive force generator flushes out the photocleaved biopolymer molecules. 
   
     
     
         25 .- 27 . (canceled)