Novel peptide derived from pep27 peptide and uses thereof

Abstract

A peptide according to an embodiment of the present in which, in the amino acid sequence of SEQ ID NO: 1, (i) 2nd and 4th amino acids are each substituted with tryptophan (W); (ii) 2nd, 4th, 11st and 13rd amino acids are each substituted with tryptophan; and (iii) 2nd, 4th, 19th, 20th, 22nd, 23rd, 26th and 27th amino acids are each substituted with tryptophan. The peptide can be usefully applied as an active ingredient for antibiotics, cosmetic compositions, food additives, feed additives, biological pesticides and quasi-drugs.

What is claimed is: 
     
         1 . A peptide in which, in the amino acid sequence of SEQ ID NO: 1,
 (i) 2 nd  and 4 th  amino acids are each substituted with tryptophan (W);   (ii) 2 nd , 4 th , 11 st  and 13 rd  amino acids are each substituted with tryptophan; or   (iii) 2 nd , 4 th , 19 th , 20 th , 22 nd , 23 rd , 26 th  and 27 th  amino acids are each substituted with tryptophan.   
     
     
         2 . The peptide according to  claim 1 , wherein the peptide consists of the amino acid sequence of SEQ ID NO: 2. 
     
     
         3 . The peptide according to  claim 1 , wherein the peptide consists of the amino acid sequence of SEQ ID NO: 3. 
     
     
         4 . The peptide according to  claim 1 , wherein the peptide consists of the amino acid sequence of SEQ ID NO: 4. 
     
     
         5 . An antimicrobial composition comprising the peptide according to  claim 1  as an active ingredient. 
     
     
         6 . The antimicrobial composition according to  claim 5 , wherein the antimicrobial composition is a pharmaceutical composition. 
     
     
         7 . The antimicrobial composition according to  claim 5 , wherein the antimicrobial composition is any one selected from the group consisting of a cosmetic compositions, a food additive, a feed additive, a biological pesticide, an antiseptic composition and a quasi-drug compositions. 
     
     
         8 . A method of killing or reducing a growth of a microorganism comprising at least one selected from the group consisting of gram-positive bacteria, gram-negative bacteria and antibiotics-tolerant bacteria, the method comprising administering a composition comprising the peptide of  claim 1  to a subject in need thereof. 
     
     
         9 . The method of  claim 8 , wherein the microorganism comprises the gram-positive bacteria selected from the group consisting of  Starphylococcus aureus, Bacillus subtilis, Listeria monocytogenes  and a combination thereof. 
     
     
         10 . The method of  claim 8 , wherein the microorganism comprises the gram-negative bacteria selected from the group consisting of  Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium  and a combination thereof. 
     
     
         11 . The method of  claim 8 , wherein the microorganism comprises the antibiotics-tolerant bacteria selected from the group consisting of  Starphylococcus aureus  ( S. aureus ),  Escherichia coli  ( E. coli ),  Pseudomonas aeruginosa  ( P. aeruginosa ),  Salmonella typhimurium  ( S. typhimurium ), and a combination thereof, which have antibiotic tolerance. 
     
     
         12 . The method of  claim 8 , wherein the peptide consists of the amino acid sequence of SEQ ID NO: 2. 
     
     
         13 . The method of  claim 8 , wherein the peptide consists of the amino acid sequence of SEQ ID NO: 3. 
     
     
         14 . The method of  claim 8 , wherein the peptide consists of the amino acid sequence of SEQ ID NO: 4.