Target Gene-Detecting Device and Method for Detecting Target Gene, Using Same

Abstract

The present invention relates to a target gene-detecting device and a method for detecting a target gene. According to an aspect, a target gene-detecting device can be conveniently fabricated at low cost by employing a porous substrate or a method for detecting a target gene allows the pretreatment of a sample, the extraction of a nucleic acid, the amplification of a nucleic acid, and the detection of a target gene to be conducted at high accuracy and specificity in an integral system, with no contamination plausibility and can be thus useful for gene inspection.

1 . A target gene-detecting device comprising:
 a first porous substrate supporting a composition for nucleic acid detection; and   a second porous substrate having a metal nanostructure,   wherein the first porous substrate and the second porous substrate are arranged to be spaced a predetermined distance apart from each other by a connection unit,   wherein the connection unit totally or partially connects the first porous substrate and the second porous substrate to each other.   
     
     
         2 . The device of  claim 1 , wherein the composition for nucleic acid detection comprises a cell-lysing composition, a composition for protein degradation, a composition for suppressing nucleic acid degradation, or a combination thereof. 
     
     
         3 . The device of  claim 1 , wherein the metal nanostructure comprises gold, silver, platinum, aluminum, iron, zinc, bronze, brass, nickel, alloys of these metals, or a combination thereof. 
     
     
         4 . The device of  claim 1 , wherein the metal nanostructure is a metal nanoparticle, a metal nanorod, a metal nanoisland, a metal nanocap, a metal nanowire, a metal nanocrescent moon, a metal nanorice, a metal nanoshell, a metal nanostar, or a combination thereof. 
     
     
         5 . The device of  claim 1 , wherein the second porous substrate supports a nucleic acid-amplifying composition. 
     
     
         6 . The device of  claim 1 , wherein the second porous substrate comprises a composition for nucleic acid detection. 
     
     
         7 . The device of  claim 1 , wherein a material of the first porous substrate or the second porous substrate is paper, nylon, nitrocellulose (NC), polyester (PE), polysulfonate (PS), polyether sulfone (PES), polytetrafluoroethylene (PTFE), polyvinylidene fluoride (PVDF), polypropylene (PP), cellulose, cellulose acetate (CA), regenerated cellulose (RC), glass, or a combination thereof. 
     
     
         8 . The device of  claim 1 , which emits a signal of light scattered after being irradiated with a laser.