Compositions and Methods for Control of Insect Pests

Abstract

Novel insecticidal proteins that are toxic to lepidopteran pests are disclosed. The polynucleotides encoding the insecticidal proteins can be used to transform prokaryotic and eukaryotic organisms to express the insecticidal proteins. The recombinant organisms or compositions containing the recombinant organisms or the insecticidal proteins alone or in combination with other pest control agents and an appropriate agricultural carrier can be used to control lepidopteran pests in various environments.

1 . A recombinant Cry protein that is toxic to a lepidopteran pest, wherein the recombinant Cry protein comprises (a) an amino acid sequence that has at least 99% sequence identity with an amino acid sequence of SEQ ID NO: 3. 
     
     
         2 . (canceled) 
     
     
         3 . The recombinant Cry protein of  claim 2 , wherein the recombinant Cry protein comprises SEQ ID NO:3, SEQ ID NO:6, or a toxin fragment of SEQ ID NO:3 or SEQ ID NO:6. 
     
     
         4 . The recombinant Cry protein of  claim 3 , wherein the lepidopteran pest is selected from the group consisting of European corn borer ( Ostrinia nubilalis ), fall armyworm ( Spodoptera frugiperda ), corn earworm ( Helicoverpa zea ), sugarcane borer ( Diatraea saccharalis ), velvetbean caterpillar ( Anticarsia gemmatalis ), soybean looper ( Chrysodeixis includens ), southwest corn borer ( Diatraea grandiosella ), western bean cutworm ( Richia albicosta ), tobacco budworm ( Heliothis virescens ), Asian corn borer ( Ostrinia furnacalis ), cotton bollworm ( Helicoverpa armigera ), striped stem borer ( Chilo suppressalis ), pink stem borer ( Sesamia calamistis ) and rice leaffolder ( Cnaphalocrocis medinalis ). 
     
     
         5 . A polynucleotide comprising a nucleotide sequence that a) encodes a recombinant Cry protein of  claim 1 ; or b) comprises any of SEQ ID NOs:14-29, or toxin-encoding fragments thereof. 
     
     
         6 . A chimeric gene comprising a heterologous promoter that functions in a plant or bacteria operably linked to the polynucleotide of  claim 5 . 
     
     
         7 . The chimeric gene of  claim 6 , wherein a) the plant expressible promoter is a ubiquitin, cestrum yellow virus, corn TrpA, OsMADS 6, maize H3 histone, bacteriophage T3 gene 9 5′ UTR, corn sucrose synthetase 1, corn alcohol dehydrogenase 1, corn light harvesting complex, corn heat shock protein, maize mtl, pea small subunit RuBP carboxylase, rice actin, rice cyclophilin, Ti plasmid mannopine synthase, Ti plasmid nopaline synthase, petunia chalcone isomerase, bean glycine rich protein 1, potato patatin, lectin, CaMV 35S or a S-E9 small subunit RuBP carboxylase promoter; or b) the bacteria expressible promoter comprises nucleotides 12-197 of SEQ ID NO:30. 
     
     
         8 . An insecticidal composition comprising the recombinant Cry protein of  claim 1  wherein the composition comprises a bacterium or a plant comprising the recombinant Cry protein. 
     
     
         9 . A recombinant vector comprising the polynucleotide of  claim 5 . 
     
     
         10 . A transgenic bacterial cell or plant cell comprising the recombinant vector of  claim 9 . 
     
     
         11 . The transgenic plant cell of  claim 10 , wherein the plant cell is a) a dicot plant cell; or b) a monocot plant cell; or c) a dicot plant cell selected from the group consisting of a soybean cell, sunflower cell, tomato cell, cole crop cell, cotton cell, sugar beet cell and tobacco cell; or d) a monocot plant cell selected from the group consisting of a barley cell, maize cell, oat cell, rice cell, sorghum cell, sugar cane cell and wheat cell. 
     
     
         12 . A transgenic plant comprising the transgenic plant cell of  claim 11 . 
     
     
         13 . A transgenic seed of the transgenic plant of  claim 12 , wherein the transgenic seed comprises the polynucleotide. 
     
     
         14 . A harvested product derived from seed of  claim 13 , wherein the harvested product comprises the recombinant Cry protein and the recombinant Cry protein has the same function it had in the transgenic plant. 
     
     
         15 . A processed product derived from the harvested product of  claim 14 , wherein the processed product is selected from the group consisting of flour, meal, oil, and starch, or a product derived therefrom, and wherein the processed product comprises the recombinant Cry protein and the recombinant Cry protein has the same function it had in the transgenic plant. 
     
     
         16 . A method of producing a recombinant Cry protein that is toxic to a lepidopteran pest comprising: culturing the transgenic cell of  claim 10  under conditions in which the transgenic cell produces the recombinant Cry protein. 
     
     
         17 . A method of producing an insect-resistant transgenic plant comprising: introducing into a plant the chimeric gene of  claim 6 , wherein the recombinant Cry protein is expressed in the plant, thereby producing an insect-resistant transgenic plant. 
     
     
         18 . The method of  claim 17 , wherein the introducing step is achieved by a) transforming the plant with the chimeric gene; or b) crossing a first plant comprising the chimeric gene with a different second plant; or c) genome editing a chimeric gene preexisting in a transgenic plant. 
     
     
         19 . A method of controlling a lepidopteran pest, comprising delivering to the lepidopteran pest or an environment thereof a composition comprising an effective amount of the recombinant Cry protein of  claim 1 . 
     
     
         20 . (canceled)