Molecular marker combination linked to quantitative traits of tea plant (+)-catechin content

Abstract

A molecular marker combination linked to quantitative traits of tea plant (+)-catechin content, including a SNP site 1, a SNP site 2, a SNP site 3, a SNP site 4, a SNP site 5, a SNP site 6, a SNP site 7 and a SNP site 8, which are located in tea genomes Scaffold4239:309117, Scaffold3614: 66549, Scaffold349: 3413816, Scaffold1989: 2316385, Scaffold451: 940283, Scaffold3727:442660, Scaffold115:803980 and Scaffold920:281727, respectively, and genotypes thereof are extremely significantly correlated with the (+)-catechin content is provided. A detection method for detecting each site, and one or more molecular marker site is used to evaluate the tea plant (+)-catechin content.

1 . A molecular marker combination linked to quantitative traits of tea plant (+)-catechin content, comprising a SNP site 1, a SNP site 2, a SNP site 3, a SNP site 4, a SNP site 5, a SNP site 6, a SNP site 7 and a SNP site 8, which are located in tea genomes Scaffold4239:309117, Scaffold3614: 66549, Scaffold349: 3413816, Scaffold1989: 2316385, Scaffold451: 940283, Scaffold3727:442660, Scaffold115:803980 and Scaffold920:281727, respectively, which are a 501st base of a nucleotide sequence shown in SEQ ID NO:1, a 501st base of a nucleotide sequence shown in SEQ ID NO:4, a 501st base of a nucleotide sequence shown in SEQ ID NO:7, a 501st base of a nucleotide sequence shown in SEQ ID NO:10, a 501st base of a nucleotide sequence shown in SEQ ID NO:13, a 501st base of a nucleotide sequence shown in SEQ ID NO:16, a 501st base of a nucleotide sequence shown in SEQ ID NO:19, and a 501st base of a nucleotide sequence shown in SEQ ID NO:22. 
     
     
         2 . A method of using of any one or more molecular marker of the molecular marker combination according to  claim 1  in evaluating the tea plant (+)-catechin content. 
     
     
         3 . A method of using of primers of any one or more molecular marker of the molecular marker combination according to  claim 1  in evaluating the tea plant (+)-catechin content. 
     
     
         4 . Primers for detecting the SNP site 1 according to  claim 1 , wherein the primers have nucleotide sequences shown as SEQ ID NO: 2 and SEQ ID NO: 3. 
     
     
         5 . Primers for detecting the SNP site 2 according to  claim 1 , wherein the primers have nucleotide sequences shown as SEQ ID NO: 5 and SEQ ID NO: 6. 
     
     
         6 . Primers for detecting the SNP site 3 according to  claim 1 , wherein the primers have nucleotide sequences shown as SEQ ID NO: 8 and SEQ ID NO: 9. 
     
     
         7 . Primers for detecting the SNP site 4 according to  claim 1 , wherein the primers have nucleotide sequences shown as SEQ ID NO: 11 and SEQ ID NO: 12. 
     
     
         8 . Primers for detecting the SNP site 5 according to  claim 1 , wherein the primers have nucleotide sequences shown as SEQ ID NO: 14 and SEQ ID NO: 15. 
     
     
         9 . Primers for detecting the SNP site 6 according to  claim 1 , wherein the primers have nucleotide sequences shown as SEQ ID NO: 17 and SEQ ID NO: 18. 
     
     
         10 . Primers for detecting the SNP site 5 according to  claim 1 , wherein the primers have nucleotide sequences shown as SEQ ID NO: 20 and SEQ ID NO: 21. 
     
     
         11 . Primers for detecting the SNP site 6 according to  claim 1 , wherein the primers have nucleotide sequences shown as SEQ ID NO: 23 and SEQ ID NO: 24. 
     
     
         12 . A kit for evaluating tea plant (+)-catechin content, comprising a reagent for detecting the molecular marker combination according to  claim 1  or any one molecular marker thereof. 
     
     
         13 . The kit according to  claim 12 , wherein the reagent is selected from one or more of the following primers: primers for detecting the SNP site 1 which have nucleotide sequences shown as SEQ ID NO: 2 and SEQ ID NO: 3, primers for detecting the SNP site 2 which have nucleotide sequences shown as SEQ ID NO: 5 and SEQ ID NO: 6, primers for detecting the SNP site 3 which have nucleotide sequences shown as SEQ ID NO: 8 and SEQ ID NO: 9, primers for detecting the SNP site 4 which have nucleotide sequences shown as SEQ ID NO: 11 and SEQ ID NO: 12, primers for detecting the SNP site 5 which have nucleotide sequences shown as SEQ ID NO: 14 and SEQ ID NO: 15, primers for detecting the SNP site 6 which have nucleotide sequences shown as SEQ ID NO: 17 and SEQ ID NO: 18, primers for detecting the SNP site 5 which have nucleotide sequences shown as SEQ ID NO: 20 and SEQ ID NO: 21, and primers for detecting the SNP site 6 which the primers have nucleotide sequences shown as SEQ ID NO: 23 and SEQ ID NO: 24. 
     
     
         14 . A method for evaluating tea plant (+)-catechin content, wherein the method detects a genotype of any one or more molecular markers of the molecular marker combination according to  claim 1 . 
     
     
         15 . A method of using of one or more of any one or more molecular markers of the molecular marker combination according to  claim 1  in molecular-assisted breeding. 
     
     
         16 . A method of using of primers in molecular-assisted breeding, wherein the primers are selected from: primers for detecting the SNP site 1 which have nucleotide sequences shown as SEQ ID NO: 2 and SEQ ID NO: 3, primers for detecting the SNP site 2 which have nucleotide sequences shown as SEQ ID NO: 5 and SEQ ID NO: 6, primers for detecting the SNP site 3 which have nucleotide sequences shown as SEQ ID NO: 8 and SEQ ID NO: 9, primers for detecting the SNP site 4 which have nucleotide sequences shown as SEQ ID NO: 11 and SEQ ID NO: 12, primers for detecting the SNP site 5 which have nucleotide sequences shown as SEQ ID NO: 14 and SEQ ID NO: 15, primers for detecting the SNP site 6 which have nucleotide sequences shown as SEQ ID NO: 17 and SEQ ID NO: 18, primers for detecting the SNP site 5 which have nucleotide sequences shown as SEQ ID NO: 20 and SEQ ID NO: 21, and primers for detecting the SNP site 6 which the primers have nucleotide sequences shown as SEQ ID NO: 23 and SEQ ID NO: 24. 
     
     
         17 . A method of using of the kit according to  claim 12  in molecular-assisted breeding.