Recombinant collagen and elastin molecules and uses thereof

Abstract

This disclosure provides non-naturally occurring collagen and elastin molecules. The non-naturally occurring collagens and elastins include truncated collagens, truncated elastins, as well as fusion proteins thereof. The non-naturally occurring collagen and elastin are useful in foods, cosmetics and many other products and uses.

1 .- 25 . (canceled) 
     
     
         26 . A recombinant polypeptide produced in a microbial host cell, the recombinant polypeptide comprising an amino acid sequence of full-length human type 21 alpha 1 collagen having a truncation of from 50 amino acids to 800 amino acids, wherein the truncation comprises a truncation at the N-terminal end and the C-terminal end of the full-length human type 21 alpha 1 collagen, wherein the recombinant polypeptide comprises the amino acid sequence according to SEQ ID NO: 76, and wherein the recombinant polypeptide is monomeric and does not form a stable triple helix structure of natural collagen. 
     
     
         27 . The recombinant polypeptide of  claim 26 , wherein the truncation further comprises an internal truncation of the full-length human type 21 alpha 1 collagen. 
     
     
         28 . The recombinant polypeptide of  claim 26 , wherein the truncation comprises a truncation at the C-terminal end of the full-length human type 21 alpha collagen from 50 amino acids and 250 amino acids. 
     
     
         29 . The recombinant polypeptide of  claim 26 , wherein the truncation comprises a truncation at the N-terminal end of the full-length human type 21 alpha 1 collagen of between 50 amino acids and 600 amino acids. 
     
     
         30 . The recombinant polypeptide of  claim 26 , wherein the recombinant polypeptide consists of the amino acid sequence according to SEQ ID NO: 76. 
     
     
         31 . The recombinant polypeptide of  claim 26 , further comprising one or more selected from the group consisting of: a secretion tag, a histidine tag, a fluorescent protein, a protease cleavage site, and a beta-lactamase protein. 
     
     
         32 . The recombinant polypeptide of  claim 31 , wherein the secretion tag is DsbA. 
     
     
         33 . A composition comprising from 0.005% w/w to 30% w/w of a recombinant polypeptide of  claim 26 . 
     
     
         34 . The composition of  claim 33 , wherein the recombinant polypeptide comprises SEQ ID NO: 76. 
     
     
         35 . The composition of  claim 33 , wherein the recombinant polypeptide consists of SEQ ID NO: 76. 
     
     
         36 . The composition of  claim 33 , wherein the composition is formulated for topical application. 
     
     
         37 . The composition of  claim 36 , wherein the composition comprises one or more of a topical carrier, a preservative, or both. 
     
     
         38 . The composition of  claim 37 , wherein the topical carrier is selected from the group consisting of: a liposome, a biodegradable microcapsule, a lotion, a spray, an aerosol, a dusting powder, a biodegradable polymer, a mineral oil, a triglyceride oil, a silicone oil, glycerin, glycerin monostearate, an alcohol, an emulsifying agents, a liquid petroleum, a white petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene, wax, sorbitan monostearate, polysorbate, cetyl ester wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol, cyclomethicone, cyclopentasiloxane, water, and any combination thereof. 
     
     
         39 . The composition of  claim 37 , wherein the preservative is selected from the group consisting of: tocopherol, diiodomethyl-p-tolylsulfone, 2-Bromo-2-nitropropane-1,3-diol, cis isomer 1-(3-chloroallyl)-3,5,7-triaza-1-azoniaadamantane chloride, glutaraldehyde, 4,4-dimethyl oxazolidine, 7-Ethylbicyclooxazolidine, methyl paraben, sorbic acid, Germaben II, rosemary extract, EDTA, and any combination thereof. 
     
     
         40 . The composition of  claim 36 , wherein the composition is a cosmetic. 
     
     
         41 . A method of treating the skin of a subject or providing a cosmetic benefit to the skin of the subject, the method comprising administering or applying to the skin of a subject a composition of  claim 36 , thereby treating the skin of the subject or providing the cosmetic benefit to the skin of the subject. 
     
     
         42 . The method of  claim 41 , wherein the administering or the applying results in an increase in viability of keratinocytes, fibroblasts, or both, of the subject after exposure to UV radiation. 
     
     
         43 . The method of  claim 41 , wherein the administering or the applying results in an increase in viability of keratinocytes, fibroblasts, or both, of the subject after exposure to urban dust. 
     
     
         44 . The method of  claim 41 , wherein the administering or the applying results in a decrease in production of inflammatory cytokines. 
     
     
         45 . A recombinant cell comprising at least one copy of a heterologous nucleic acid sequence encoding a recombinant polypeptide according to  claim 26 . 
     
     
         46 . The recombinant cell of  claim 45 , wherein the recombinant cell is a bacterial cell. 
     
     
         47 . The recombinant cell of  claim 46 , wherein the bacterial cell is  Escherichia coli.    
     
     
         48 . The recombinant cell of  claim 45 , wherein the heterologous nucleic acid sequence comprises SEQ ID NO: 75. 
     
     
         49 . The recombinant cell of  claim 45 , wherein the heterologous nucleic acid sequence is codon-optimized for expression in a cell. 
     
     
         50 . The recombinant cell of  claim 45 , wherein the recombinant cell is capable of secreting the recombinant polypeptide extracellularly. 
     
     
         51 . A composition comprising the recombinant cell of  claim 45 , and a culture media comprising a recombinant polypeptide of  claim 26 . 
     
     
         52 . A method for producing a recombinant polypeptide, the method comprising:
 a) incubating a recombinant cell of  claim 45  in a culture media, wherein the recombinant cell secretes the recombinant polypeptide into the culture media;   b) collecting the culture media comprising the recombinant polypeptide secreted thereto; and   c) purifying the recombinant polypeptide from the culture media.