Materials and methods for serial multiplexed detection of rna in cells and tissues

Abstract

This disclosure describes materials and methods for effectively performing serial multiplexed FISH analysis.

What is claimed is: 
     
         1 . An article of manufacture, comprising
 at least one DNA probe set, wherein the at least one DNA probe set binds to a set of RNAs; and   RNase H or DNase.   
     
     
         2 . The article of manufacture of  claim 1 , further comprising:
 reagents for hybridizing, selectively, the at least one DNA probe set to the set of RNAs.   
     
     
         3 . The article of manufacture of  claim 1 , further comprising a second DNA probe set, wherein the second DNA probe set binds to a second set of RNAs. 
     
     
         4 . The article of manufacture of  claim 3 , further comprising a third probe set, wherein the third DNA probe set binds to a third set of RNAs. 
     
     
         5 . The article of manufacture of  claim 4 , further comprising a fourth probe set, wherein the fourth DNA probe set binds to a fourth set of RNAs. 
     
     
         6 . The article of manufacture of  claim 5 , further comprising a fifth probe set, wherein the fifth DNA probe set binds to a fifth set of RNAs. 
     
     
         7 . The article of manufacture of  claim 2 , wherein the reagents for hybridizing, selectively, the at least one DNA probe set to the set of RNAs comprise reagents suitable for use in a branched DNA (bDNA) RNA-single molecule (sm) fluorescent in situ hybridization (FISH) method. 
     
     
         8 . The article of manufacture of  claim 1 , wherein the at least one DNA probe set comprises at least one DNA oligonucleotide probe. 
     
     
         9 . The article of manufacture of  claim 1 , wherein the at least one DNA probe set comprises a plurality of DNA oligonucleotide probes. 
     
     
         10 . The article of manufacture of  claim 9 , wherein each of the plurality of DNA oligonucleotide probes in the at least one DNA probe set comprises a differentially detectable label.